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Table 5 Physiological data derived from steady-state chemostat cultures of engineered l-arabinose-metabolizing S. cerevisiae strains

From: The Penicillium chrysogenum transporter PcAraT enables high-affinity, glucose-insensitive l-arabinose transport in Saccharomyces cerevisiae

 

IMX929 (GAL2)

IMX1508 (PcaraT)

Residual l-arabinose (g L−1)

1.77 ± 0.19

0.004 ± 0.002

YX/S [g biomass (g l-arabinose)−1]

0.48 ± 0.06

0.40 ± 0.01

ql-arabinose (mmol g−1 h−1)

0.70 ± 0.10

0.80 ± 0.08

  1. Strains expressing either GAL2 (IMX929) or PcaraT (IMX1508) as the sole functional l-arabinose transporter were grown in aerobic, l-arabinose-limited chemostat cultures (7.5 g L−1 l-arabinose, dilution rate = 0.05 h−1, pH 5, T = 30 °C). Data are derived from independent triplicate experiments and presented as average ± mean deviation