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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

Fig. 2

Design of the synthetic genes for Golden Gate gene shuffling. a BsaI recognition sites between two modules are flanked and mirrored. The four nucleotides at the end of one fragment are replicated at the beginning of the next one to avoid nucleotide loss after the ligation. b Donor vectors have the same fragment arrangement. The wild-type fragments are represented in green, while the fragments in orange have one mutation site each. The dotted sections (pUK57 backbone and BsaI sites) are lost after the ligation. The shuffled library consists of pBAD SUMO-HMFO vectors with a random combination and correct order of the 7 gene modules (with mutations)

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