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Table 1 Steady-state parameters of the different HMFO variants on HMF

From: Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

HMFO

kcat (s−1)

Km (mM)

kcat/Km (s−1 mM−1)

WT

13.7

1.52

9.03

7xHMFO

11.8

0.63

18.7

  1. Kinetic parameters were determined by measuring H2O2 formation in a coupled assay using HMF as substrate (Additional file 4: Figure S1)
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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