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Table 1 Steady-state parameters of the different HMFO variants on HMF

From: Creating a more robust 5-hydroxymethylfurfural oxidase by combining computational predictions with a novel effective library design

HMFO kcat (s−1) Km (mM) kcat/Km (s−1 mM−1)
WT 13.7 1.52 9.03
7xHMFO 11.8 0.63 18.7
  1. Kinetic parameters were determined by measuring H2O2 formation in a coupled assay using HMF as substrate (Additional file 4: Figure S1)