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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: H2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum

Fig. 5

Central metabolism flux levels and relative protein expression of high biomass gas-fermenting C. autoethanogenum chemostats. Data for high biomass concentration chemostats (~ 1.4 gDCW/L) are shown. See dashed inset for bar chart and heatmap details. Fluxes (mmol/gDCW/h) are represented as the average ± standard deviation between duplicate (syngas) and quadruplicate (CO and high-H2 CO) chemostats. Arrows show the direction of calculated fluxes; red arrow denotes uptake or secretion. Flux into PEP from OAA and pyruvate is merged. Refer to Additional file 1: Fig. S5 for the cofactors of the reactions used in the model and Additional file 2: Table S2 for metabolite abbreviations. Protein expression fold changes are average of quadruplicate chemostats: syngas vs. CO (left box) and high-H2 CO vs. CO (right box). SIL-protein-aided label-based data are denoted with red font for gene ID. Differentially expressed proteins are indicated with an asterisk (q value < 0.05 after false discovery rate [FDR] correction [58], and for label-free data additionally fold-change > 1.5). Proteins forming a complex are highlighted with orange borders; FdhA (13725) forms a complex with HytA–E (13745–13770) for direct CO2 reduction with H2. Median data are shown for the Rnf and ATPase protein complexes. aMethylene-THF reductase flux is shown; bbifunctional acetaldehyde/alcohol dehydrogenase (acetyl-CoA → ethanol). Gene IDs next to heatmaps are preceded with CAETHG_RS; gDCW gram dry cell weight, NQ not quantified. Refer to Additional file 3: Tables S3 and S4 for flux data and Additional file 4: Tables S5–S7 for protein expression data

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