Fig. 3
From: Mn2+ modulates the expression of cellulase genes in Trichoderma reesei Rut-C30 via calcium signaling
TPHO84-1 and TPHO84-2 regulate cellulase production of T. reesei Rut-C30 with Mn2+ supplementation. a The hyphal growth of T. reesei Rut-C30 and its derivative mutant strains under 0 or 10 mM MnCl2. b The measurement of colony diameter. The CMCase activity (c) and pNPCase activity (d) of T. reesei Rut-C30 and its derivative mutant strains were examined after culture in medium containing 0 or 10 mM MnCl2. The transcriptional levels of cbh1 (e) and egl1 (f) in the parental strain Rut-C30 and its derivative mutant strains were analyzed after cultured in medium containing 0 or 10 mM MnCl2. Values are the mean ± SD of the results from three independent experiments. Asterisks indicate significant differences (*p < 0.05, Student’s t test)