Fig. 5

Effects of Ca²⁺ channel inhibitor LaCl₃ on the cytosolic Ca²⁺ concentration and cellulase production. A Fluorescence analysis of LaCl₃ influence on cytosolic Ca²⁺ burst induced by Mn²⁺. The T. reesei Rut-C30 were cultured in liquid minimal medium for 48–60 h with 0 or 10 mM MnCl₂ (0 or 10 Mn, respectively), and then treated with 0 or 5 mM LaCl₃. For detection, 50 μM Fluo-3/AM was used, and the intensity was monitored using Automatic Inverted Fluorescence Microscopy. Green fluorescence represents the free cytosolic Ca²⁺. DIC, differential interference contrast, CK, not treated with LaCl₃. B Comparative fluorescence ratio analysis of LaCl₃ influence on the cytosolic Ca²⁺ burst induced by Mn²⁺. The y-axis represents the Ca²⁺ fluorescence ratio measured by CLSM and the x-axis the different treatments. The CMCase activity (C) and pNPCase activity (D) of T. reesei Rut-C30 were examined after culture in medium containing 0 or 10 mM MnCl₂ and with (−) or without (+) 5 mM LaCl₃. The expression levels of cbh1 (E) and egl1 (F) in T. reesei Rut-C30 were analyzed after culture in medium containing 0 or 10 mM MnCl₂ and with (−) or without (+) 5 mM LaCl₃. Values are the mean ± SD of the results from three independent experiments. Different letters indicate significant differences between the columns (p < 0.05, according to Duncan’s multiple-range test)