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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Uncovering the molecular mechanisms of lignocellulose digestion in shipworms

Fig. 1

Overview of the anatomy, lignocellulolytic activities and digestive meta-transcriptome of Lyrodus pedicellatus. a Schematic diagram of L. pedicellatus, showing cecum, gills and the two portions (anterior and posterior) of the digestive gland. b In vitro activity assay of cecum fluids with a panel of polysaccharides, determined via DNS assay [8]. The detected activities on glucans, mannans and xylans are generally compatible with the putative function of the shipworm GHs based on sequence similarity to characterized proteins. P pachyman, PASC phosphoric acid swollen cellulose, CMC carboxy methyl cellulose, βG beta-glucan, L lichenan, X xylan, AX arabinoxylan, M mannan, GM glucomannan, GaM galactomannan, LBG locust bean gum, Ga galactan. Bars indicate means (error bars: standard deviations of three replicates). c Compositional analysis of lignocellulose fractions (Cell cellulose, Hemi hemicellulose, Lign lignin) from Scots pine before (“wood”) and after passing through the shipworm digestive system (“frass”). Bars indicate means (error bars: standard deviations of five replicates). d, e, f Pie charts showing the relative transcript abundance (obtained from TPM values, see “Methods” for more details) of CAZymes identified in digestive gland (d), cecum (e) and gills (f) of L. pedicellatus. Enzyme families where all members are of bacterial origin are marked with a pound sign (#)

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