|
Enzyme
|
Substrate
|
Glycosidic linkage
|
Vmax (Ua mg−1 protein)
|
Km (mM)
|
kcat (s−1)
|
kcat/Km (mM−1 s−1)
|
|---|
|
Bgl1B
|
Laminaribiose
|
β-1,3
|
1.1 × 102
|
9.9 × 10−1
|
9.1 × 101
|
9.2 × 101
|
|
Cellobiose
|
β-1,4
|
1.6 × 101
|
2.0 × 101
|
1.3 × 101
|
6.5 × 10−1
|
|
Novozyme 188
|
Laminaribiose
|
β-1,3
|
4.2 × 10−1
|
2.5 × 100
|
8.5 × 10−1
|
3.4 × 10−1
|
|
Cellobiose
|
β-1,4
|
3.9 × 100
|
3.4 × 100
|
7.8 × 100
|
2.3 × 100
|
- Kinetic parameters: each value of parameter is the mean of experimental triplicates
- aOne unit (U) of Bgl1B was defined as the amount of enzyme required to produce 1 μmol of glucose per min from 0.1% (w/v) cellobiose in 20 mM sodium phosphate buffer (pH 6.0) at 40 °C
