Fig. 2

Conversion of 200 µM 2,6-DMP and hydrocoerulignone by 2 µM NcLPMO9C in 100 mM sodium succinate/phosphate buffer, pH 6.0. a Reaction of 2,6-DMP in the presence of 100 µM H₂O₂ and 250 µM dissolved O₂, and b in the presence of 250 µM O₂ only, followed for 5000 s. Time frequency between each trace is 1000 s. c Spectra of 2,6-DMP (black line) and hydrocoerulignone (blue line), 200 µM each. Inset demonstrates the conversion of hydrocoerulignone; note that the occurring peak is similar to a, followed for 300 s. Time frequency between each trace is 50 s. d Time course of the NcLPMO9C (0.3 µM)-catalyzed conversion of 1 mM 2,6-DMP (black line) and 1 mM hydrocoerulignone (blue line) measured at 469 nm in the presence of 100 µM H₂O₂ in 100 mM sodium succinate/phosphate buffer, pH 6.0. The data are expressed as mean values (± SD), from three independent repeats