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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: A fast and sensitive activity assay for lytic polysaccharide monooxygenase

Fig. 4

Reaction stoichiometry. Calculated concentrations of converted 2,6-DMP (top graphs) or hydrocoerulignone (bottom graphs) after oxidation with NcLPMO9C, laccase, and horseradish peroxidase. Experiments with setting different H2O2 concentrations (left graphs) or setting different concentrations of 2,6-DMP or hydrocoerulignone (right graphs). a Linear increase of converted 2,6-DMP at pH 7.5 until 20 µM for NcLPMO9C (black diamonds) or 30 µM for horseradish peroxidase (green circle). b Linear increase of converted 2,6-DMP at pH 6.0 (blue triangle) and pH 7.5 (cyan triangles) until 30 µM for laccase without addition of H2O2. c Linear increase of converted hydrocoerulignone at pH 6.0 and d at pH 7.5 until 5 µM for NcLPMO9C (black diamonds; with addition of 100 µM H2O2) and pH 6.0 for laccase (blue triangle; without addition of H2O2). The data are expressed as mean values (± SD), from at least three independent repeats. Linear range was taken to calculate the molar absorption coefficient for coerulignone (ε469 = 53,200 M−1 cm−1). Dashed line represents the ideal 1:1 stoichiometry for the 2,6-DMP:H2O2 ratio based on the molar absorption coefficient

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