A fast and sensitive activity assay for lytic polysaccharide monooxygenase

Table 1 Kinetic constants of NcLPMO9C for H₂O₂ and 2,6-DMP

	Kinetic constants of NcLPMO9C for H₂O₂			Kinetic constants of NcLPMO9C for 2,6-DMP
	Cosubstrate 2,6-DMP (mM)	K_M (µM)	V_max (U g⁻¹)	Cosubstrate H₂O₂ (µM)	K_M (mM)	V_max (U g⁻¹)
pH 6.0	1	34.3 ± 2.4	7.5 ± 0.2	25	245 ± 74	270 ± 20
	10	39.8 ± 2.5	32.6 ± 0.8	100	100 ± 13	235 ± 15
	25	41.1 ± 2.7	59.5 ± 1.4	300	144 ± 28	352 ± 43
pH 7.5	0.5	7.3 ± 0.9	22.3 ± 0.6	25	172 ± 49	2500 ± 400
	1.0	10.7 ± 1.1	32.5 ± 0.8	100	132 ± 32	2250 ± 250
	2.0	11.8 ± 0.6	50.2 ± 1.1	300	124 ± 31	2300 ± 300

The constants were determined for pH 6.0 and 7.5 and for three different cosubstrate concentrations. It is obvious that for 2,6-DMP no saturation was achieved and therefore the kinetic constants for H₂O₂ were not determined under pseudo-first-order conditions

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