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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Lipid productivity in limnetic Chlorella is doubled by seawater added with anaerobically digested effluent from kitchen waste

Fig. 3

Images under (a) a fluorescence microscope and (b) a confocal microscope, and fluorescence intensity (c) for Nile Red-stained neutral lipid in Chlorella sorokiniana SDEC-18 cultivated in BG11 and seawater supplemented with different volume percentages (0 and 3%) of anaerobically digested effluent from kitchen waste. Shown in Fig. 3b are hydrocarbon oils stained using the neutral lipid-binding stain Nile Red (yellow) and chlorophyll autofluorescence (red). An individual cell was defined by chlorophyll autofluorescence from the chloroplast in each cell. Each image is an overlay of Nile Red signal, chlorophyll autofluorescence signal, and a bright-field image. Scale bar, 20 μm

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