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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Water-splitting-based, sustainable and efficient H2 production in green algae as achieved by substrate limitation of the Calvin–Benson–Bassham cycle

Fig. 2

Photosynthetic electron transport during H2 production of Chlamydomonas in acetate-free HS medium induced by dark anaerobic incubation. a, b H2 and net O2 productions upon the addition of the PSII inhibitor DCMU, and DMSO, used as a control. c, d H2 and net O2 productions upon the addition of the respiratory inhibitors potassium cyanide (KCN) and salicylhydroxamic acid (SHAM). e, f H2 and net O2 productions upon the addition of 2% CO2. g, h H2 and net O2 productions upon the addition of the CBB cycle inhibitor glycolaldehyde (GA). i, j H2 and net O2 productions upon the addition of and the ionophore carbonylcyanide p-triflouromethoxyphenylhydrazone (FCCP). The time points at which the various treatments were applied are indicated by arrow. In the right Y axis, the percentage of the corresponding gases in the headspaces of the cultures are shown. All experiments were carried out at 320 µmol photons/m2/s following the 4-h dark anaerobic incubation (0 time point). Mean values (± SEM) are each based on 4 or 5 biological replicates

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