Fig. 5
The effects of an iron-salt-based O2 absorbent on the hydrogenase activity of Chlamydomonas in acetate-free HS medium induced by dark anaerobic incubation. a HYDA1 expression level, expressed relative to the aerobically cultured controls in the presence and absence of the O2 absorbent. b The amount of HydA, determined by western blot analysis in the presence and absence of the O2 absorbent; samples of 2 µg chl (a + b) were loaded and the second to fourth lanes (25, 50, 100% of 0 h control) are for approximate quantitation of HydA. The 0 h control samples were collected directly after the 4-h dark anaerobic incubation. c The in vitro hydrogenase activity in the presence and absence of the O2 absorbent, expressed as a percentage of the activity of cells subjected to dark anaerobic incubation for 3 h, which had an in vitro hydrogenase activity of approx. 500 µmol H2/mg chl (a + b)/h. Mean values (± SEM) are each based on 3 or 4 biological replicates