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Table 1 Light-to-H2 energy conversion efficiency in Chlamydomonas reinhardtii in HS medium under various conditions

From: Water-splitting-based, sustainable and efficient H2 production in green algae as achieved by substrate limitation of the Calvin–Benson–Bassham cycle

Conditions of H2 production Time of illumination following dark anaerobic incubation (h)
0.25 1 3 5 24
Control (HS)
 H2 produced (µl/ml) 3.46 3.86 8.41 10.80 27.47
 Efficiency (%) 1.43 0.40 0.29 0.22 0.11
+ Glc + GO + Asc
 H2 produced (µl/ml) n.d. 38.57 85.33 92.68 117.04
 Efficiency (%) n.d. 4.00 2.95 1.92 0.51
+ O2 absorbent
 H2 produced (µl/ml) 3.85 6.73 13.09 19.20 62.10
 Efficiency (%) 1.60 0.70 0.45 0.40 0.27
  1. Algal cultures containing 50 µg chl (a + b)/ml and dark-incubated for 4 h were illuminated using cool white fluorescent tubes, at an intensity of 52 W/m2, corresponding to approx. 320 µmol photons/m2/s. See “Materials and methods” for the calculations on the incident light-to-H2 conversion efficiency. The experiments were carried out in HS medium (control) and the effects of a combined glucose (Glc, 2 mM), glucose oxidase (GO, 0.2 mg/ml) and ascorbate (Asc, 1 mM) treatment and the effects of an iron-salt O2 absorbent were also assessed
  2. nd non-determined