Fig. 3

Alignment of FjoAcXE with structurally and biochemically characterized SGNH hydrolase-type esterases. RGAE: rhamnogalacturonan acetylesterase from Aspergillus aculeatus (PDB code 1DEO) [52]; TAP: Thioesterase I/Protease I/Lysophospholipase L1 from E. coli (PDB code 1IVN) [53, 54]; Sm23: arylesterase from Sinorhizobium meliloti (PDB code 4TX1) [55]; CjCE2A and CjCE2B: acetylxylan esterase from Cellvibrio japonicus (PDB codes 2WAA and2W9X, respectively) [43]; CtCE2: acetylxylan esterase from Ruminiclostridium thermocellum (PDB code 2WAO) [43]; Est2A: acetylxylan esterase from Butyrivibrio proteoclasticus (PDB code 3U37) [56]. Conserved SGNH residues (green triangle) and catalytic Ser-His dyad or Ser-Asp-His triad (red squares) are highlighted. Sequences were aligned using MAFFT v.7.017 [57]