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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: A novel acetyl xylan esterase enabling complete deacetylation of substituted xylans

Fig. 5

Activity of FjoAcXE on acetylated monosaccharides and xylooligosaccharides. Reactions (30 µL) comprised 0.5 µg of FjoAcXE in 50 mM HEPES (pH 8.0) and 0.1% (w/v) of β-d-glucose pentaacetate (G), β-d-xylopyranose tetraacetate (X), 1,2,3,4-tetra-O-acetyl-l-rhamnopyranose (R), or acetylated xylooligosaccharides from Eucalyptus (Ac-XOS); reactions were incubated at 30 °C for 20 min. Release of acetic acid was measured using acetic acid kit (K-ACETRM; Megazyme). n = 3; error bars correspond to standard deviation

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