Fig. 1

LC–MS chromatograms of enzymatically released sodium adduct of aldouronic acids. Charged product profiles generated from treatment of LRP with either CuGE, GH10 endo-xylanase or a combination of the two (presented on the same intensity scale and displayed with an offset between enzyme combinations). The product profiles show a mixture of acetylated aldouronic acids containing 4-O-methyl-glucuronosyl (MeGlcA) ranging from DP 3 to DP 5. Each product is assigned with a number and overall compound composition according to the molecular mass. Several compound masses gave rise to several peaks, i.e., compound (5) indicating structural isomers as a result of differences in substitution pattern. The zoom in the upper right corner shows an enlargement of the product profile resulting from treatment with CuGE alone