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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: The natural catalytic function of CuGE glucuronoyl esterase in hydrolysis of genuine lignin–carbohydrate complexes from birch

Fig. 3

Products released from hydrolysis of LRP by GH10 endo-xylanase and CuGE. a Total amounts of aldotriuronic acid (MeGlcAXyl2), aldotetrauronic acid (MeGlcAXyl3), and aldopentauronic acid (MeGlcAXyl4) released by GH10 endo-xylanase and CuGE on LRP quantified relative to reduced aldotetrauronic acid by LC–MS. The theoretical sum of products released by GH10 and CuGE together is calculated as a sum of the products released by the individual enzymes. Dotted lines indicate the level of the theoretical sum on the actual observed release of products. Total amounts of MeGlcA in molar equivalents originating from the aldouronic acids are represented as a scatter with black markers on the secondary axis. The total MeGlcA concentration in the lignin-rich precipitate is illustrated by an orange horizontal line on the secondary axis (for details on quantification see Additional file 2). b Total amounts of xylose and major xylo-oligosaccharides (DP2-DP4) released by GH10 endo-xylanase and CuGE on LRP. Xylose concentration quantified by HPAEC-PAD and xylo-oligos (DP2-DP4) quantified on LC–MS. Black scatter markers referring to the secondary axis represent the calculated total release in xylose mole equivalents originating from aldouronic acids, xylose, and xylo-oligos. Total amount of xylose in LRP after acid hydrolysis is indicated by an orange horizontal line. All quantifications are performed in triplicate and depicted with standard deviations. Calculated MeGlcA and xylose equivalents are depicted with pooled standard deviations

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