Engineering of E. coli inherent fatty acid biosynthesis capacity to increase octanoic acid production

Table 1 Metabolic engineering of E. coli existing fatty acid biosynthesis pathway for fatty acid production in minimal medium

Fatty acid	Strain	Genetic modifications	Thioesterase	Culture condition	Titer (g/L)	Yield (mg/g glucose)	Productivity (mg/L/h)	Source
MCFA (C12–C18)	BL21	ΔfadL	TesA’	Bioreactor, minimal medium with 2% (wt/v) glucose	4.8	44	126	[15]
	BL21		AbTE	Bioreactor, M9 medium with 0.5% tryptone and feeding of glucose	3.6	61	89	[19]
	BL21	ΔfadD, +acc	TesA’ + CcTE	Bioreactor, M9 with feeding of glycerol	2.5	48	170	[16]
	MG1655	ΔfadD, +fabZ	RcTE	Shake flask, M9 with 1.5% glucose	1.7	113	35	[27]
	DH1	ΔfadE	TesA’	Shake flask, minimal with 2% glucose	3.8	190	53	[50]
	DH1	+fadR	TesA’	Shake flask, minimal with 2% glucose	5.2	260	72	[51]
	BL21	Modular optimization of multi-genes	CnFatB2	Bioreactor, MK with 1% YE and feeding of glucose	8.6	78	124	[18]
Octanoate (C8)	K27	ΔfadD	Various	Culture tube, LB-grown preculture was resuspended in M9 with 0.4% glucose	0.18^a	48.0^a	10.0^a	[24]
	BL21 (DE3)	ΔfadD Δpta ΔlacY fabF^mut fabBDeg	CpFatB1	96-well plate, LB-grown preculture was diluted 1:20 in M9 with 0.5% glucose	0.24^a	45.0^a	4.5^a	[17]
	MG1655	ΔfadD	AtTE	Bioreactor, MOPS with 2% glucose	0.044	4.7	0.45	[26]
	MG1655	ldhA::M1-93-pssA, mgsA::M1-93-acrAB, maeB::M1-93-tolC	AtTE	Shake flask, MOPS with 2% glucose	0.22	20.0	3.1	[25]
	MG1655	mgsA::M1-93-fabZ ΔfadE ΔfumAC ΔackA	AtTE	Shake flask, M9 with 1.5% glucose	0.44	30.0	6.2	This study
	MG1655	Same as above	AtTE	Bioreactor, M9 with 1.5% glucose	0.50	33.3	10.4	This study
	MG1655	Same as above	AtTE	Bioreactor, M9 with 2.63% glucose	1.0	38.0	10.4	This study

TE, thioesterase; TesA’, cytosolic E. coli TE 1; FatB, plant fatty acyl-ACP thioesterase; Ab, Acinetobacter baylyi; Cc, Cinnamomum camphorum; Rc, Ricinus communis; Cn, Cocos nucifera; Cp, Cuphea palustris; At, Anaerococcus tetradius; YE, yeast extract
^aUsing LB as preculture

ISSN: 2731-3654