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Table 1 Metabolic engineering of E. coli existing fatty acid biosynthesis pathway for fatty acid production in minimal medium

From: Engineering of E. coli inherent fatty acid biosynthesis capacity to increase octanoic acid production

Fatty acid Strain Genetic modifications Thioesterase Culture condition Titer (g/L) Yield (mg/g glucose) Productivity (mg/L/h) Source
MCFA (C12–C18) BL21 ΔfadL TesA’ Bioreactor, minimal medium with 2% (wt/v) glucose 4.8 44 126 [15]
BL21   AbTE Bioreactor, M9 medium with 0.5% tryptone and feeding of glucose 3.6 61 89 [19]
BL21 ΔfadD, +acc TesA’ + CcTE Bioreactor, M9 with feeding of glycerol 2.5 48 170 [16]
MG1655 ΔfadD, +fabZ RcTE Shake flask, M9 with 1.5% glucose 1.7 113 35 [27]
DH1 ΔfadE TesA’ Shake flask, minimal with 2% glucose 3.8 190 53 [50]
DH1 +fadR TesA’ Shake flask, minimal with 2% glucose 5.2 260 72 [51]
BL21 Modular optimization of multi-genes CnFatB2 Bioreactor, MK with 1% YE and feeding of glucose 8.6 78 124 [18]
Octanoate (C8) K27 ΔfadD Various Culture tube, LB-grown preculture was resuspended in M9 with 0.4% glucose 0.18a 48.0a 10.0a [24]
BL21 (DE3) ΔfadD Δpta ΔlacY fabFmut fabBDeg CpFatB1 96-well plate, LB-grown preculture was diluted 1:20 in M9 with 0.5% glucose 0.24a 45.0a 4.5a [17]
MG1655 ΔfadD AtTE Bioreactor, MOPS with 2% glucose 0.044 4.7 0.45 [26]
MG1655 ldhA::M1-93-pssA, mgsA::M1-93-acrAB, maeB::M1-93-tolC AtTE Shake flask, MOPS with 2% glucose 0.22 20.0 3.1 [25]
MG1655 mgsA::M1-93-fabZ ΔfadE ΔfumAC ΔackA AtTE Shake flask, M9 with 1.5% glucose 0.44 30.0 6.2 This study
MG1655 Same as above AtTE Bioreactor, M9 with 1.5% glucose 0.50 33.3 10.4 This study
MG1655 Same as above AtTE Bioreactor, M9 with 2.63% glucose 1.0 38.0 10.4 This study
  1. TE, thioesterase; TesA’, cytosolic E. coli TE 1; FatB, plant fatty acyl-ACP thioesterase; Ab, Acinetobacter baylyi; Cc, Cinnamomum camphorum; Rc, Ricinus communis; Cn, Cocos nucifera; Cp, Cuphea palustris; At, Anaerococcus tetradius; YE, yeast extract
  2. aUsing LB as preculture