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Table 3 Effects of combinatorial implementation of different interventions on free octanoic acid production

From: Engineering of E. coli inherent fatty acid biosynthesis capacity to increase octanoic acid production

Strain Time (h) Cell density (OD550) Glucose used (g/L) Acetate titer (g/L) C8 titer (mg/L) C8 yield (mg/g glucose) C8 productivity (mg/L/h) Total FFAs (mg/L)
MG1655 (TE10) 72 1.91 ± 0.1 13.8 ± 0.2 0.63 ± 0.02 275 ± 12 19.9 ± 0.9 3.81 ± 0.17 315 ± 14
+fabZ (TE10) 72 4.51 ± 0.2 14.8 ± 0.1 1.8 ± 0.2 398 ± 8.3 26.9 ± 0.6 5.53 ± 0.12 479 ± 22
+fabZ ΔfadE (TE10) 72 4.62 ± 0.1 14.8 ± 0.1 1.7 ± 0.02 387 ± 12 26.2 ± 0.8 5.38 ± 0.16 462 ± 14
+fabZ ΔfadB (TE10) 72 4.76 ± 0.3 14.8 ± 0.1 1.3 ± 0.3 395 ± 8.2 26.7 ± 0.5 5.49 ± 0.11 469 ± 12
+fabZ ΔfadA (TE10) 72 1.90 ± 0.1 13.2 ± 0.4 0.9 ± 0.2 300 ± 5.7 22.7 ± 0.4 4.17 ± 0.079 350 ± 6.2
+fabZ ΔfadE ΔfumAC (TE10) 72 4.74 ± 0.2 14.4 ± 0.2 1.1 ± 0.2 408 ± 4.8 28.3 ± 0.3 5.67 ± 0.066 515 ± 8.8
+fabZ ΔfadE ΔfumAC ΔackA (TE10) 72 5.38 ± 0.1 14.8 ± 0.1 1.4 ± 0.1 442 ± 14 30.0 ± 0.8 6.15 ± 0.19 615 ± 21
+fabZ ΔfadE ΔfumAC Δpta (TE10) 72 4.79 ± 0.03 14.6 ± 0.1 1.4 ± 0.3 408 ± 4.9 27.2 ± 0.3 5.67 ± 0.068 532 ± 5.9