Table 3 Effects of combinatorial implementation of different interventions on free octanoic acid production
Strain | Time (h) | Cell density (OD550) | Glucose used (g/L) | Acetate titer (g/L) | C8 titer (mg/L) | C8 yield (mg/g glucose) | C8 productivity (mg/L/h) | Total FFAs (mg/L) |
|---|---|---|---|---|---|---|---|---|
MG1655 (TE10) | 72 | 1.91 ± 0.1 | 13.8 ± 0.2 | 0.63 ± 0.02 | 275 ± 12 | 19.9 ± 0.9 | 3.81 ± 0.17 | 315 ± 14 |
+fabZ (TE10) | 72 | 4.51 ± 0.2 | 14.8 ± 0.1 | 1.8 ± 0.2 | 398 ± 8.3 | 26.9 ± 0.6 | 5.53 ± 0.12 | 479 ± 22 |
+fabZ ΔfadE (TE10) | 72 | 4.62 ± 0.1 | 14.8 ± 0.1 | 1.7 ± 0.02 | 387 ± 12 | 26.2 ± 0.8 | 5.38 ± 0.16 | 462 ± 14 |
+fabZ ΔfadB (TE10) | 72 | 4.76 ± 0.3 | 14.8 ± 0.1 | 1.3 ± 0.3 | 395 ± 8.2 | 26.7 ± 0.5 | 5.49 ± 0.11 | 469 ± 12 |
+fabZ ΔfadA (TE10) | 72 | 1.90 ± 0.1 | 13.2 ± 0.4 | 0.9 ± 0.2 | 300 ± 5.7 | 22.7 ± 0.4 | 4.17 ± 0.079 | 350 ± 6.2 |
+fabZ ΔfadE ΔfumAC (TE10) | 72 | 4.74 ± 0.2 | 14.4 ± 0.2 | 1.1 ± 0.2 | 408 ± 4.8 | 28.3 ± 0.3 | 5.67 ± 0.066 | 515 ± 8.8 |
+fabZ ΔfadE ΔfumAC ΔackA (TE10) | 72 | 5.38 ± 0.1 | 14.8 ± 0.1 | 1.4 ± 0.1 | 442 ± 14 | 30.0 ± 0.8 | 6.15 ± 0.19 | 615 ± 21 |
+fabZ ΔfadE ΔfumAC Δpta (TE10) | 72 | 4.79 ± 0.03 | 14.6 ± 0.1 | 1.4 ± 0.3 | 408 ± 4.9 | 27.2 ± 0.3 | 5.67 ± 0.068 | 532 ± 5.9 |