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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by site-directed mutagenesis on loop 6

Fig. 3

Enzymatic properties of the wild-type GtCel5 and its variants. a pH–activity profiles tested at the optimal temperature of each enzyme with 10 mg/mL CMC-Na as the substrate in 100 mM citric acid–Na2HPO4 buffer over the pH range of 2.2–7.0 for 10 min. b Temperature–activity profiles tested at the optimal pH of each enzyme in the temperature range of 40–90 °C for 10 min. c pH–stability profiles determined by measuring the residual activity at optimal pH (100 mM citric acid–Na2HPO4) and temperature for 10 min after 1-h incubation at pH 1.0–12.0 and 37 °C without substrate. d Temperature–stability profiles investigated by measuring the residual activity at optimal pH and temperature after incubation at different temperatures for various durations

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