Table 1 The kinetic values of GtCel5, TeEgl5A, PoCel5, and their mutants with CMC-Na as the substrate
Enzymes | Specific activity (U/mg) | Km (mg/mL) | Vmax (μmol/min/mg) | kcat (/s) | kcat/Km (mL/s/mg) |
|---|---|---|---|---|---|
GtCel5 | 1117 ± 43 | 4.5 ± 0.3 | 1475 ± 71 | 878 ± 44 | 195 ± 6 |
N233G | 1901 ± 21 | 3.6 ± 0.4 | 1802 ± 96 | 1072 ± 63 | 297 ± 8 |
N233A | 1419 ± 22 | 3.7 ± 0.3 | 1742 ± 96 | 1036 ± 57 | 283 ± 4 |
N233D | 1185 ± 35 | 4.4 ± 0.7 | 1511 ± 98 | 899 ± 87 | 202 ± 5 |
N233S | 1039 ± 26 | 7.5 ± 0.2 | 2302 ± 112 | 1370 ± 74 | 184 ± 3 |
TeEgl5A | 732 ± 10 | 7.4 ± 0.3 | 1014 ± 54 | 600 ± 24 | 81.1 ± 7.5 |
TeEgl5A_G216A | 523 ± 30 | 10.9 ± 0.4 | 1255 ± 94 | 732 ± 43 | 61.2 ± 6.7 |
TeEgl5A_G216N | 368 ± 15 | 11.3 ± 0.6 | 958 ± 76 | 559 ± 33 | 49.4 ± 4.3 |
PoCel5 | 401 ± 3 | 5.6 ± 0.3 | 534 ± 28 | 311 ± 37 | 55.1 ± 5.2 |
PoCel5_G210A | 374 ± 2 | 5.5 ± 0.4 | 599 ± 36 | 337 ± 43 | 62.5 ± 12.1 |
PoCel5_G210N | 166 ± 4 | 6.9 ± 0.6 | 280 ± 32 | 163 ± 24 | 23. 7 ± 3.2 |