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Table 1 The kinetic values of GtCel5, TeEgl5A, PoCel5, and their mutants with CMC-Na as the substrate

From: Enhancing the catalytic activity of a novel GH5 cellulase GtCel5 from Gloeophyllum trabeum CBS 900.73 by site-directed mutagenesis on loop 6

Enzymes Specific activity (U/mg) Km (mg/mL) Vmax (μmol/min/mg) kcat (/s) kcat/Km (mL/s/mg)
GtCel5 1117 ± 43 4.5 ± 0.3 1475 ± 71 878 ± 44 195 ± 6
N233G 1901 ± 21 3.6 ± 0.4 1802 ± 96 1072 ± 63 297 ± 8
N233A 1419 ± 22 3.7 ± 0.3 1742 ± 96 1036 ± 57 283 ± 4
N233D 1185 ± 35 4.4 ± 0.7 1511 ± 98 899 ± 87 202 ± 5
N233S 1039 ± 26 7.5 ± 0.2 2302 ± 112 1370 ± 74 184 ± 3
TeEgl5A 732 ± 10 7.4 ± 0.3 1014 ± 54 600 ± 24 81.1 ± 7.5
TeEgl5A_G216A 523 ± 30 10.9 ± 0.4 1255 ± 94 732 ± 43 61.2 ± 6.7
TeEgl5A_G216N 368 ± 15 11.3 ± 0.6 958 ± 76 559 ± 33 49.4 ± 4.3
PoCel5 401 ± 3 5.6 ± 0.3 534 ± 28 311 ± 37 55.1 ± 5.2
PoCel5_G210A 374 ± 2 5.5 ± 0.4 599 ± 36 337 ± 43 62.5 ± 12.1
PoCel5_G210N 166 ± 4 6.9 ± 0.6 280 ± 32 163 ± 24 23. 7 ± 3.2