Fig. 1

Phenotype and genotype of Trichoderma reesei QM9414 disruption mutants. The β-glucosidase activities A and cellulase activities B of three T. reesei mutant strains bearing a randomly integrated amdS marker gene (i.e., QM9414_Dhax1_8 (D8), _10 (D10) and _14 (D14); yellow bars) and their parent strain QM9414 (PS; brown bars). Strains were cultivated on xylan for 66 h (for β–glucosidase assays) and on lactose for 48 h (for cellulase assays). Reaction with the Azo-cellazyme C tablet for detection of cellulase activity was performed for 90 min. Enzymatic activities are given in μ and referred to biomass (dry weight). The values are means of biological triplicates. The error bars depict the standard deviation and different letters denote statistical difference among compared data employing ANOVA (P < 0.05). C Southern blot analysis using SacII-digested chromosomal DNA of the investigated strains and a locus-specific probe. Expected signals are at 5178 bp (from integration of the amdS cassette at this locus), 2960 bp (for the native locus) and 2124 bp (for a sequence downstream of the amdS targeted locus)