Fig. 5

Cellulase activities of complemented QM9414_Dhax1 strains. Cultivation of QM9414_Dhax1 strains overexpressing either hax1_QM6a (D/OE QM6a; blue bars), hax1_QM9414 (D/OE QM9414; yellow bars) or hax1_Rut-C30 (D/OE Rut-C30; purple bars) and the respective parent strains QM9414_Dhax1_8 and _14 (QM D8 and QM D14; grey bars) was performed on lactose for 72 h. A Cellulase activities of parent strains and the corresponding overexpression strains after cultivation on lactose for 48 h are given in reference to the culture volume. B Cellulase activities of the parent strain QM9414_Dhax1_14 and the corresponding overexpression strains after cultivation on lactose for 37, 48, 61 and 72 h are given in reference to the culture volume. c Cellulase activities of the parent strain QM9414_Dhax1_14 and the corresponding overexpression strains after cultivation on lactose for 72 h are given in reference to the final biomass (dry weight). Reaction with the Azo-Cellazyme C tablet for detection of cellulase activity was performed for 2 h, 45, 20, and 20 min for the timepoints 37, 48, 61, and 72 h, respectively. All values are means of biological triplicates. The error bars depict the standard deviation and different letters denote statistical difference among compared data employing ANOVA (P < 0.05)