Fig. 6

Cellulase activities of QM6a hax1 overexpression strains. Cultivation of QM6a overexpressing either hax1_QM6a (OE QM6a; blue bars), hax1_QM9414 (OE QM9414; yellow bars) or hax1_Rut-C30 (OE Rut-C30; purple bars), the parent strain QM6a_∆tmus53 (QM6a; light grey bars) and QM6a_∆tmus53_∆pyr4 as a reference for a pyr4 deleted background (Dpyr4; dark grey bars) was performed on lactose for 72 h. A Cellulase activities of the overexpression and reference strains after cultivation on lactose for 37, 48, 61 and 72 h are given in reference to the culture volume. B Cellulase activities of the overexpression and reference strains after cultivation on lactose for 72 h are given in reference to the final biomass (dry weight). Reaction with the Azo-Cellazyme C tablet for detection of cellulase activity was performed for 5, 3, 2 h and 75 min for the timepoints 37, 48, 61, and 72 h, respectively. All values are means of biological triplicates. The error bars depict the standard deviation and different letters denote statistical difference among compared data employing ANOVA (P < 0.05)