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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Efficient whole cell biocatalyst for formate-based hydrogen production

Fig. 1

H2 production from formate by resting cells of A. woodii. Cells were grown with 20 mM fructose, harvested in the exponential growth phase, and suspended in buffer (50 mM imidazole, 20 mM KCl, 20 mM MgSO4, 4 mM DTE, pH 7) to a final protein concentration of 1 mg mL−1 (corresponding to a CDW of 2.3 g L−1) in anoxic serum bottles (gas phase 100% N2). The bottles were incubated in a shaking water bath at 30 °C. At the beginning of the experiment, sodium formate, potassium formate, ETH2120, NaCl, and ethanol (solvent of ETH2120 as negative control) were added as indicated. Triangles down, 300 mM sodium formate, 30 µM ETH2120 (dissolved in 100% ethanol), 20 mM NaCl; diamonds, 300 mM sodium formate, 20.5 mM ethanol, 20 mM NaCl; circles, 100 mM K-formate; triangles up, 100 mM sodium formate, 20.5 mM ethanol, 20 mM NaCl

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