Fig. 2
From: Efficient whole cell biocatalyst for formate-based hydrogen production
Influence of the growth substrate (a) and pH (b) on H2 production. a Cells were grown with 20 mM fructose (squares), 2 atm. H2 + CO2 (80:20 [v:v], triangles), or 100 mM sodium formate (circles). The experiment was performed as described for Fig. 1 using 300 mM sodium formate, 30 µM ETH2120, and 20 mM NaCl. b Fructose-grown cells were suspended in buffer (25 mM MES, 25 mM Tris, 25 mM MOPS, 25 mM CHES, 20 mM KCl, 20 mM MgSO4, 4 mM DTE, 20 mM NaCl) at pH 6 (circles), pH 7 (squares), pH 8 (triangles), pH 9 (diamonds). The experiment was started by the addition of sodium formate to a final concentration of 300 mM