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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Efficient whole cell biocatalyst for formate-based hydrogen production

Fig. 4

Influence of the formate concentration on H2 production. Cells were grown with 20 mM fructose, harvested in the exponential growth phase, and suspended in buffer (50 mM imidazole, 20 mM KCl, 20 mM MgSO4, 30 µM ETH2120, 20 mM NaCl, 4 mM DTE, pH 7) to a final protein concentration of 1 mg mL−1 (corresponding to a CDW of 2.3 g L−1). Experiments were started by the addition of 25 mM (diamonds), 50 mM (triangles down), 100 mM (triangles up), 200 mM (closed squares), 400 mM (circles), 600 mM formate (open squares)

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