Fig. 2

Effect of Tr69957 transporter on growth of S. cerevisiae in the presence of cellobiose, mannose, and xylose. a Tr69957::GFP is localized in the plasma membrane in S. cerevisiae when grown in maltose-rich medium. Microscopy images were acquired in DIC and GFP fluorescence. b Growth of S. cerevisiae strain EBY.VW4000 transformed with (Tr69957) and without (control) the transporter Tr69957 gene in the presence of 1% cellobiose, xylose, or mannose. Both the control and Tr69957 strains contained the genes encoding enzymes for xylose and cellobiose metabolic pathways. Yeast strains were initially grown in maltose-rich medium before a serial dilution was done (1:10 dilution, initial optical density OD_600nm = 1.0) and cells were incubated on plates containing the different carbon sources at 30 °C for 96 h; c cellobiose, d xylose, and e mannose transport as determined by HPLC in the S. cerevisiae Tr69957 strain in the presence of 1% of the specific carbon source. S. cerevisiae strain which did not contain the Tr69957 transporter was used as the control strain. Standard deviations were obtained from triplicate assays