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Table 2 Medium-chain alcohol tolerance of evolved strains

From: Evolutionary engineering improves tolerance for medium-chain alcohols in Saccharomyces cerevisiae

Strains µavg (h−1)*
Alcohol (%, v/v)
PrOH (3)a BuOH (1.2)a iBuOH (1.2) AOH (0.6) iAOH (0.4) PtOH (0.6)a HxOH (0.15)a HpOH (0.05)a OcOH (0.05)a
WT 0.042 ± 0.004 0.144 ± 0.014 0.195 ± 0.032 0.044 ± 0.013 0.264 ± 0.027 0 ± 0.010 0.043 ± 0.018 0 ± 0.009 0 ± 0.008
sSD003 0.172 ± 0.030# 0.170 ± 0.005* 0.202 ± 0.020 0.096 ± 0.026* 0.294 ± 0.023 0.133 ± 0.034# 0.252 ± 0.038 0.051 ± 0.026* 0.092 ± 0.009
sSD006 0.038 ± 0.004 0.134 ± 0.021 0.211 ± 0.027 0.071 ± 0.024 0.285 ± 0.024 0.145 ± 0.103 0.115 ± 0.034 0 ± 0.012 0.033 ± 0.021*
sSD019 0.129 ± 0.022# 0.140 ± 0.032 0.267 ± 0.017* 0.128 ± 0.019# 0.318 ± 0.006* 0.161 ± 0.090* 0.291 ± 0.015 0.290 ± 0.028 0.274 ± 0.063*
sSD021 0.029 ± 0.042* 0.063 ± 0.001 0.264 ± 0.022* 0.139 ± 0.028# 0.311 ± 0.017 0.260 ± 0.072# 0.217 ± 0.058 0.247 ± 0.007 0.323 ± 0.020
  1. aDifferences in growth between strains in these alcohols was only apparent at time intervals beyond the 2–10-h period used to calculate µavg for iBuOH, AOH, iAOH, and HxOH. The µavg in these alcohols was calculated for time periods when the cells were in the exponential growth phase. At these longer time interval, in tightly capped tubes, growth may not have been under fully aerobic conditions. Representative growth curves for these alcohols are given in Additional file 1: Figure S2 (PtOH) and Additional file 1: Figure S3 (HpOH)
  2. * Values are mean ± standard deviation evaluated from three biological replicates for all samples except HxOH, for which there were nine biological replicates for WT, seven for sSD003, and four each for the other three strains. Statistical significance was determined by an unpaired two-tailed Student’s t test; significant differences from the WT are indicated as follows:  P ≤ 0.001; # P ≤ 0.01; * P ≤ 0.05