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Table 3 Summary of mutations in evolved strains identified by whole-genome resequencing

From: Evolutionary engineering improves tolerance for medium-chain alcohols in Saccharomyces cerevisiae

Strain Mutation Gene Protein functionb Mutant allele
sSD003 D85E GCD1 Gamma subunit of the translation initiation factor eIF2B gcd1-1
G338C CIT2 Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle cit2-1
sSD006 R56C GCD7 Beta subunit of the translation initiation factor eIF2B gcd7-2
sSD019 D85E GCD1 Gamma subunit of the translation initiation factor eIF2B gcd1-1
G338C CIT2 Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle cit2-1
Q446a PDR5 Plasma membrane ATP-binding cassette (ABC) transporter pdr5-1
G135V UBP13 Ubiquitin-specific protease ubp13-1
S234C LSB6 Type II phosphatidylinositol 4-kinase lsb6-1
A662G NST1 Protein of unknown function; mediates sensitivity to salt stress nst1-1
Silent COX1 Subunit I of cytochrome c oxidase (Complex IV) cox1-1
sSD021 R56C GCD7 Beta subunit of the translation initiation factor eIF2B gcd7-2
D77Y SUI2 Alpha subunit of the translation initiation factor eIF2 sui2-2
G925A PDR5 Plasma membrane ATP-binding cassette (ABC) transporter pdr5-2
A529E SEY1 Dynamin-like GTPase that mediates homotypic ER fusion sey1-2
  1. aDenotes the introduction of an early stop codon
  2. bProtein function taken from the Saccharomyces genome database website http://www.yeastgenome.org