Evolutionary engineering improves tolerance for medium-chain alcohols in Saccharomyces cerevisiae

Table 3 Summary of mutations in evolved strains identified by whole-genome resequencing

Strain	Mutation	Gene	Protein function^b	Mutant allele
sSD003	D85E	GCD1	Gamma subunit of the translation initiation factor eIF2B	gcd1-1
sSD003	G338C	CIT2	Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle	cit2-1
sSD006	R56C	GCD7	Beta subunit of the translation initiation factor eIF2B	gcd7-2
sSD019	D85E	GCD1	Gamma subunit of the translation initiation factor eIF2B	gcd1-1
	G338C	CIT2	Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle	cit2-1
	Q446^a	PDR5	Plasma membrane ATP-binding cassette (ABC) transporter	pdr5-1
	G135V	UBP13	Ubiquitin-specific protease	ubp13-1
	S234C	LSB6	Type II phosphatidylinositol 4-kinase	lsb6-1
	A662G	NST1	Protein of unknown function; mediates sensitivity to salt stress	nst1-1
	Silent	COX1	Subunit I of cytochrome c oxidase (Complex IV)	cox1-1
sSD021	R56C	GCD7	Beta subunit of the translation initiation factor eIF2B	gcd7-2
	D77Y	SUI2	Alpha subunit of the translation initiation factor eIF2	sui2-2
	G925A	PDR5	Plasma membrane ATP-binding cassette (ABC) transporter	pdr5-2
	A529E	SEY1	Dynamin-like GTPase that mediates homotypic ER fusion	sey1-2

^aDenotes the introduction of an early stop codon
^bProtein function taken from the Saccharomyces genome database website http://www.yeastgenome.org

ISSN: 2731-3654