Fig. 2
Time course of the reaction of MMF (1.5 mM) with: a AAO; b AAO, UPO and H2O2; c AAO, UPO and MOX; and d AAO, UPO, MOX and methanol (at 28 °C in 100 mM phosphate, pH 7). Enzyme concentrations were 5 µM (AAO and UPO) and 1 µM (MOX), while H2O2 and methanol final concentrations (in b and d, respectively) were 1.5 mM (added after 24, 48 and 72 h) and 1 mM (added after 72 and 96 h reaction). Dashed lines in a and b represent the AAO and UPO residual activities as a function of time, respectively. Compounds were identified and quantified by GC–MS, using the estimated response factors