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Table 2 Experimental design and corresponding responses of the Box–Behnken experiment

From: Novel optimization strategy for tannase production through a modified solid-state fermentation system

Standard order A B C D Tannase activity (U/gds)
Experimental value Predicted value
1 3 3 9 3.5 3.63 3.73
2 9 3 9 3.5 4.13 4.84
3 3 9 9 3.5 10.07 9.01
4 9 9 9 3.5 14.60 14.15
5 6 6 6 1 12.46 11.91
6 6 6 12 1 11.88 11.57
7 6 6 6 6 8.57 8.53
8 6 6 12 6 8.60 8.81
9 3 6 9 1 8.70 8.70
10 9 6 9 1 12.80 12.80
11 3 6 9 6 6.77 6.61
12 9 6 9 6 8.92 8.76
13 6 3 6 3.5 6.49 5.48
14 6 9 6 3.5 11.93 12.38
15 6 3 12 3.5 5.68 5.06
16 6 9 12 3.5 11.90 12.75
17 3 6 6 3.5 7.24 8.12
18 9 6 6 3.5 10.07 10.34
19 3 6 12 3.5 6.95 7.19
20 9 6 12 3.5 11.60 11.23
21 6 3 9 1 6.48 7.07
22 6 9 9 1 13.57 13.84
23 6 9 9 6 3.24 3.47
24 6 9 9 6 11.38 11.30
25 6 6 9 3.5 12.89 13.38
26 6 6 9 3.5 13.79 13.38
27 6 6 9 3.5 13.75 13.38
28 6 6 9 3.5 13.09 13.38
29 6 6 9 3.5 13.35 13.38