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Table 2 Experimental design and corresponding responses of the Box–Behnken experiment

From: Novel optimization strategy for tannase production through a modified solid-state fermentation system

Standard order

A

B

C

D

Tannase activity (U/gds)

Experimental value

Predicted value

1

3

3

9

3.5

3.63

3.73

2

9

3

9

3.5

4.13

4.84

3

3

9

9

3.5

10.07

9.01

4

9

9

9

3.5

14.60

14.15

5

6

6

6

1

12.46

11.91

6

6

6

12

1

11.88

11.57

7

6

6

6

6

8.57

8.53

8

6

6

12

6

8.60

8.81

9

3

6

9

1

8.70

8.70

10

9

6

9

1

12.80

12.80

11

3

6

9

6

6.77

6.61

12

9

6

9

6

8.92

8.76

13

6

3

6

3.5

6.49

5.48

14

6

9

6

3.5

11.93

12.38

15

6

3

12

3.5

5.68

5.06

16

6

9

12

3.5

11.90

12.75

17

3

6

6

3.5

7.24

8.12

18

9

6

6

3.5

10.07

10.34

19

3

6

12

3.5

6.95

7.19

20

9

6

12

3.5

11.60

11.23

21

6

3

9

1

6.48

7.07

22

6

9

9

1

13.57

13.84

23

6

9

9

6

3.24

3.47

24

6

9

9

6

11.38

11.30

25

6

6

9

3.5

12.89

13.38

26

6

6

9

3.5

13.79

13.38

27

6

6

9

3.5

13.75

13.38

28

6

6

9

3.5

13.09

13.38

29

6

6

9

3.5

13.35

13.38