Strain/plasmid | Relevant characteristics | References |
---|---|---|
ATCC 13032 | C. glutamicum wild-type biotin auxotrophic | ATCC |
I-pXMJ19 | ATCC13032 (pXMJ19) | This study |
I-eco | ATCC13032 (pX-ecoAB) | This study |
I-sco | ATCC13032 (pX-scoAB) | This study |
I-ppm | ATCC13032 (pX-ppmAB) | This study |
I-xcb | ATCC13032 (pX-xcbAB) | This study |
SAZ3 | ATCC13032 ∆ldh∆ackA-pta∆pqo∆cat P sod pyc P sod ppc | Lab stock |
CGS1 | SAZ3; pX-xcbAB, pEpycgltAsucE | This study |
CGS2 | ATCC13032 ∆ldh∆ackA-pta∆pqo∆cat P sod pyc P sod ppc P sod tal P sod tkt | This study |
CGS3 | CGS2; pX-xcbAB, pEpycgltAsucE | This study |
CGS4 | ATCC13032 ∆ldh∆ackA-pta∆pqo∆cat P sod pyc P sod ppc P sod tal P sod tkt P tuf araE | This study |
CGS5 | CGS4; pX-xcbAB, pEpycgltAsucE | This study |
Plasmids | ||
 pDsacB | KanR; vector for in-frame deletion (sacB B.sub ; lacZα; OriV E.c ) | Lab stock |
 pD-ldh | pDsacB carrying the flanking sequences of the ldh gene | This study |
 pD-PSL | pDsacB carrying the sod promoter, upstream and initial sequences of the tal gene | This study |
 pD-PSK | pDsacB carrying the sod promoter, upstream and initial sequences of the tkt gene | This study |
 pD-PtufaraE | pDsacB carrying araE from B. subtilis 168 under the control of P tuf , the flanking sequences of the ldh gene | This study |
 pXMJ19 | CmR, C. glutamicum/E. coli shuttle vector (P tac , lacIq, pBL1, rrnBT2T1 terminator, OriV C.g , OriV E.c ) | Lab stock |
 pX-ecoAB | Derived from pXMJ19, for the overexpression of xylAB from E. coli MG1655 | This study |
 pX-scoAB | Derived from pXMJ19, for the overexpression of xylAB from Streptomyces coelicolor | This study |
 pX-ppmAB | Derived from pXMJ19, for the overexpression of xylAB from Paenibacillus polymyxa | This study |
 pX-xcbAB | Derived from pXMJ19, for the overexpression of xylAB from Xanthomonas campestris pv. campestris 8004 | This study |
 pEC-XK99E | KanR; C. glutamicum/E. coli shuttle vector (P trc , lacIq; pGA1, OriV C.g ., OriV E.c ) | Lab stock |
 pEpycgltAsucE | Derived from pEC-XK99E, for the overexpression of pyc, gltA and sucE | Lab stock |
 pEP2 | KanR; C. glutamicum/E. coli shuttle vector, low copy | Lab stock |
 pEP-PtufaraE | Derived from pEP2, for the overexpression of araE from B. subtilis 168 under the control of P tuf | This study |