Skip to main content

Table 1 Strains and plasmids used in this study

From: Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate

Strain/plasmid

Relevant characteristics

References

ATCC 13032

C. glutamicum wild-type biotin auxotrophic

ATCC

I-pXMJ19

ATCC13032 (pXMJ19)

This study

I-eco

ATCC13032 (pX-ecoAB)

This study

I-sco

ATCC13032 (pX-scoAB)

This study

I-ppm

ATCC13032 (pX-ppmAB)

This study

I-xcb

ATCC13032 (pX-xcbAB)

This study

SAZ3

ATCC13032 ∆ldh∆ackA-pta∆pqo∆cat P sod pyc P sod ppc

Lab stock

CGS1

SAZ3; pX-xcbAB, pEpycgltAsucE

This study

CGS2

ATCC13032 ∆ldh∆ackA-pta∆pqo∆cat P sod pyc P sod ppc P sod tal P sod tkt

This study

CGS3

CGS2; pX-xcbAB, pEpycgltAsucE

This study

CGS4

ATCC13032 ∆ldh∆ackA-pta∆pqo∆cat P sod pyc P sod ppc P sod tal P sod tkt P tuf araE

This study

CGS5

CGS4; pX-xcbAB, pEpycgltAsucE

This study

Plasmids

 pDsacB

KanR; vector for in-frame deletion (sacB B.sub ; lacZα; OriV E.c )

Lab stock

 pD-ldh

pDsacB carrying the flanking sequences of the ldh gene

This study

 pD-PSL

pDsacB carrying the sod promoter, upstream and initial sequences of the tal gene

This study

 pD-PSK

pDsacB carrying the sod promoter, upstream and initial sequences of the tkt gene

This study

 pD-PtufaraE

pDsacB carrying araE from B. subtilis 168 under the control of P tuf , the flanking sequences of the ldh gene

This study

 pXMJ19

CmR, C. glutamicum/E. coli shuttle vector (P tac , lacIq, pBL1, rrnBT2T1 terminator, OriV C.g , OriV E.c )

Lab stock

 pX-ecoAB

Derived from pXMJ19, for the overexpression of xylAB from E. coli MG1655

This study

 pX-scoAB

Derived from pXMJ19, for the overexpression of xylAB from Streptomyces coelicolor

This study

 pX-ppmAB

Derived from pXMJ19, for the overexpression of xylAB from Paenibacillus polymyxa

This study

 pX-xcbAB

Derived from pXMJ19, for the overexpression of xylAB from Xanthomonas campestris pv. campestris 8004

This study

 pEC-XK99E

KanR; C. glutamicum/E. coli shuttle vector (P trc , lacIq; pGA1, OriV C.g ., OriV E.c )

Lab stock

 pEpycgltAsucE

Derived from pEC-XK99E, for the overexpression of pyc, gltA and sucE

Lab stock

 pEP2

KanR; C. glutamicum/E. coli shuttle vector, low copy

Lab stock

 pEP-PtufaraE

Derived from pEP2, for the overexpression of araE from B. subtilis 168 under the control of P tuf

This study

  1. ATCC American type culture collection