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Table 1 Strains and plasmids used in this study

From: Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate

Strain/plasmid Relevant characteristics References
ATCC 13032 C. glutamicum wild-type biotin auxotrophic ATCC
I-pXMJ19 ATCC13032 (pXMJ19) This study
I-eco ATCC13032 (pX-ecoAB) This study
I-sco ATCC13032 (pX-scoAB) This study
I-ppm ATCC13032 (pX-ppmAB) This study
I-xcb ATCC13032 (pX-xcbAB) This study
SAZ3 ATCC13032 ∆ldhackA-ptapqocat P sod pyc P sod ppc Lab stock
CGS1 SAZ3; pX-xcbAB, pEpycgltAsucE This study
CGS2 ATCC13032 ∆ldhackA-ptapqocat P sod pyc P sod ppc P sod tal P sod tkt This study
CGS3 CGS2; pX-xcbAB, pEpycgltAsucE This study
CGS4 ATCC13032 ∆ldhackA-ptapqocat P sod pyc P sod ppc P sod tal P sod tkt P tuf araE This study
CGS5 CGS4; pX-xcbAB, pEpycgltAsucE This study
Plasmids
 pDsacB KanR; vector for in-frame deletion (sacB B.sub ; lacZα; OriV E.c ) Lab stock
 pD-ldh pDsacB carrying the flanking sequences of the ldh gene This study
 pD-PSL pDsacB carrying the sod promoter, upstream and initial sequences of the tal gene This study
 pD-PSK pDsacB carrying the sod promoter, upstream and initial sequences of the tkt gene This study
 pD-PtufaraE pDsacB carrying araE from B. subtilis 168 under the control of P tuf , the flanking sequences of the ldh gene This study
 pXMJ19 CmR, C. glutamicum/E. coli shuttle vector (P tac , lacIq, pBL1, rrnBT2T1 terminator, OriV C.g , OriV E.c ) Lab stock
 pX-ecoAB Derived from pXMJ19, for the overexpression of xylAB from E. coli MG1655 This study
 pX-scoAB Derived from pXMJ19, for the overexpression of xylAB from Streptomyces coelicolor This study
 pX-ppmAB Derived from pXMJ19, for the overexpression of xylAB from Paenibacillus polymyxa This study
 pX-xcbAB Derived from pXMJ19, for the overexpression of xylAB from Xanthomonas campestris pv. campestris 8004 This study
 pEC-XK99E KanR; C. glutamicum/E. coli shuttle vector (P trc , lacIq; pGA1, OriV C.g ., OriV E.c ) Lab stock
 pEpycgltAsucE Derived from pEC-XK99E, for the overexpression of pyc, gltA and sucE Lab stock
 pEP2 KanR; C. glutamicum/E. coli shuttle vector, low copy Lab stock
 pEP-PtufaraE Derived from pEP2, for the overexpression of araE from B. subtilis 168 under the control of P tuf This study
  1. ATCC American type culture collection