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Table 3 Succinate production from xylose by CGS1 under anaerobic conditions

From: Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate

Strain Substrate in seed culture Substrate in anaerobic fermentation Sugar consumption rate (g L−1 h−1)a Succinate yield (g g−1 sugar) Consumed sugars (g L−1) Titer (g L−1)
Succinate α-Ketoglutarate Pyruvate Lactate Fumarate Acetate
CGS1 Glucose Glucose 2.75 ± 0.03 0.81 ± 0.01 30.5 ± 0.4 24.6 ± 0.1 1.78 ± 0.01 2.19 ± 0.03 ND ND 0.46 ± 0.01
Xylose 1.39 ± 0.02 0.90 ± 0.01 30.5 ± 0.3 27.4 ± 0.3 1.34 ± 0.03 0.24 ± 0.08 ND ND 0.45 ± 0.01
Xylose Xylose 1.95 ± 0.02 0.93 ± 0.02 30.4 ± 0.3 28.2 ± 0.3 1.32 ± 0.01 0.29 ± 0.05 ND ND 1.14 ± 0.01
Glucose 2.33 ± 0.04 0.93 ± 0.01 30.1 ± 0.2 28.1 ± 0.1 2.09 ± 0.01 ND ND ND 1.69 ± 022
  1. Values are given as the averages and standard deviations of three independent cultures
  2. Succinate production was carried out with the same initial cell density (OD600 = 30). 30 g L−1 4MgCO3·Mg(OH)2·5H2O, 200 mM sodium bicarbonate, and 1 mM IPTG were added to the medium
  3. ND not detected
  4. aSugar consumption rates were calculated at 8 h before the sugars were completely depleted