Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate

Table 3 Succinate production from xylose by CGS1 under anaerobic conditions

Strain	Substrate in seed culture	Substrate in anaerobic fermentation	Sugar consumption rate (g L⁻¹ h⁻¹)^a	Succinate yield (g g⁻¹ sugar)	Consumed sugars (g L⁻¹)	Titer (g L⁻¹)
Strain	Substrate in seed culture	Substrate in anaerobic fermentation	Sugar consumption rate (g L⁻¹ h⁻¹)^a	Succinate yield (g g⁻¹ sugar)	Consumed sugars (g L⁻¹)	Succinate	α-Ketoglutarate	Pyruvate	Lactate	Fumarate	Acetate
CGS1	Glucose	Glucose	2.75 ± 0.03	0.81 ± 0.01	30.5 ± 0.4	24.6 ± 0.1	1.78 ± 0.01	2.19 ± 0.03	ND	ND	0.46 ± 0.01
	Glucose	Xylose	1.39 ± 0.02	0.90 ± 0.01	30.5 ± 0.3	27.4 ± 0.3	1.34 ± 0.03	0.24 ± 0.08	ND	ND	0.45 ± 0.01
	Xylose	Xylose	1.95 ± 0.02	0.93 ± 0.02	30.4 ± 0.3	28.2 ± 0.3	1.32 ± 0.01	0.29 ± 0.05	ND	ND	1.14 ± 0.01
	Xylose	Glucose	2.33 ± 0.04	0.93 ± 0.01	30.1 ± 0.2	28.1 ± 0.1	2.09 ± 0.01	ND	ND	ND	1.69 ± 022

Values are given as the averages and standard deviations of three independent cultures
Succinate production was carried out with the same initial cell density (OD₆₀₀ = 30). 30 g L⁻¹ 4MgCO₃·Mg(OH)₂·5H₂O, 200 mM sodium bicarbonate, and 1 mM IPTG were added to the medium
ND not detected
^aSugar consumption rates were calculated at 8 h before the sugars were completely depleted

ISSN: 2731-3654