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Table 4 Succinate production from sugar mixtures with different ratios of glucose to xylose by CGS5 under anaerobic conditions

From: Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate

Strain Succinate yield Consumed sugars (g L−1) Titer (g L−1)
(g/g total sugars) Glucose Xylose Succinate α-Ketoglutarate Pyruvate Lactate Fumarate Acetate
CGS5 0.98 ± 0.01 0 30.4 ± 0.3 29.7 ± 0.1 1.41 ± 0.01 0.31 ± 0.01 ND ND 0.66 ± 0.01
0.98 ± 0.03 10.6 ± 0.1 21.1 ± 0.2 30.9 ± 0.6 1.04 ± 0.36 0.24 ± 0.09 ND ND 0.65 ± 0.02
0.97 ± 0.05 15.7 ± 0.3 15.0 ± 0.4 29.7 ± 0.9 0.70 ± 0.09 0.19 ± 0.04 ND ND 0.59 ± 0.02
1.00 ± 0.03 21.6 ± 0.1 10.1 ± 0.1 31.8 ± 0.3 1.24 ± 0.06 0.27 ± 0.01 ND ND 0.63 ± 0.01
1.00 ± 0.01 31.5 ± 0.1 0 31.4 ± 0.1 0.80 ± 0.02 0.38 ± 0.01 ND ND 0.52 ± 0.01
  1. Values are given as the averages and standard deviations of three independent cultures
  2. Succinate production was carried out with the same initial cell density (OD600 = 30). 30 g L−1 4MgCO3·Mg(OH)2·5H2O, 200 mM sodium bicarbonate, and 1 mM IPTG were added to the medium
  3. ND not detected