Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate

Table 4 Succinate production from sugar mixtures with different ratios of glucose to xylose by CGS5 under anaerobic conditions

Strain	Succinate yield	Consumed sugars (g L⁻¹)		Titer (g L⁻¹)
Strain	(g/g total sugars)	Glucose	Xylose	Succinate	α-Ketoglutarate	Pyruvate	Lactate	Fumarate	Acetate
CGS5	0.98 ± 0.01	0	30.4 ± 0.3	29.7 ± 0.1	1.41 ± 0.01	0.31 ± 0.01	ND	ND	0.66 ± 0.01
	0.98 ± 0.03	10.6 ± 0.1	21.1 ± 0.2	30.9 ± 0.6	1.04 ± 0.36	0.24 ± 0.09	ND	ND	0.65 ± 0.02
	0.97 ± 0.05	15.7 ± 0.3	15.0 ± 0.4	29.7 ± 0.9	0.70 ± 0.09	0.19 ± 0.04	ND	ND	0.59 ± 0.02
	1.00 ± 0.03	21.6 ± 0.1	10.1 ± 0.1	31.8 ± 0.3	1.24 ± 0.06	0.27 ± 0.01	ND	ND	0.63 ± 0.01
	1.00 ± 0.01	31.5 ± 0.1	0	31.4 ± 0.1	0.80 ± 0.02	0.38 ± 0.01	ND	ND	0.52 ± 0.01

Values are given as the averages and standard deviations of three independent cultures
Succinate production was carried out with the same initial cell density (OD₆₀₀ = 30). 30 g L⁻¹ 4MgCO₃·Mg(OH)₂·5H₂O, 200 mM sodium bicarbonate, and 1 mM IPTG were added to the medium
ND not detected

ISSN: 2731-3654