Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate

Mao, Yufeng; Li, Guiying; Chang, Zhishuai; Tao, Ran; Cui, Zhenzhen; Wang, Zhiwen; Tang, Ya-jie; Chen, Tao; Zhao, Xueming

doi:10.1186/s13068-018-1094-z

Biotechnology for Biofuels and Bioproducts

Table 5 Production of succinate from non-food lignocellulosic biomass

From: Metabolic engineering of Corynebacterium glutamicum for efficient production of succinate from lignocellulosic hydrolysate

Strain	Substrate (treatment)	Conditions	Titer (g/L)	Yield (g/g)	Productivity (g L⁻¹ h⁻¹)	References
Actinobacillus succinogenes
CGMCC1593	Corn straw (enzymatic hydrolysis)	One stage, anaerobic fed-batch fermentation, complex medium	53.2	0.83	1.21	[48]
CGMCC1593	Corn stover (enzymatic hydrolysis)	One stage, anaerobic batch fermentation, complex medium	47.4	0.72^a	0.99	[49]
NJ113	Corn fiber (dilute acid hydrolysis)	One stage, anaerobic batch fermentation, complex medium	35.4	0.73	0.98	[50]
NJ113	Sugarcane bagasse (dilute acid hydrolysis)	One stage, anaerobic batch fermentation, complex medium	23.7	0.79	0.99	[51]
NJ113	Corn fiber hydrolysate (dilute acid hydrolysis)	One stage, anaerobic batch fermentation, complex medium	47.6	0.68	0.63	[9]
NJ113	Corn stover (enzymatic hydrolysis)	One stage, anaerobic batch fermentation, complex medium	56.4	0.73	1.08	[52]
130Z	Corn stover (dilute acid hydrolysis, deacetylation)	One stage, anaerobic continuous fermentation, complex medium	39.6	0.78	1.77	[53]
130Z	Corn stover (dilute acid hydrolysis, deacetylation)	One stage, anaerobic batch fermentation, complex medium	42.8	0.74	1.27	[54]
130Z	Pinewood (enzymatic hydrolysis)	One stage, anaerobic batch fermentation, complex medium	20.7	0.65	0.90	[55]
CIP 106512	Sugarcane bagasse hemicellulose (dilute acid hydrolysis)	One stage, anaerobic batch fermentation, complex medium	22.5	0.43	1.01	[56]
Escherichia coli
BA204	Corn stalk (dilute acid hydrolysis)	Two stages, aerobic culture of biomass and anaerobic batch fermentation, complex medium	11.1	1.02	0.70	[7]
AFP 184	Spruce softwood (dilute acid hydrolysis)	Two stages, aerobic culture of biomass and anaerobic batch fermentation, complex medium	42.2	0.72	0.78	[57]
SD121	Corn stalk (enzymatic hydrolysis)	Two stages, aerobic culture of biomass and anaerobic batch fermentation, complex medium	57.8	0.87	0.96	[8]
BA305	Sugarcane bagasse (enzymatic hydrolysis)	Two-stages, aerobic culture of biomass and anaerobic batch fermentation, Cell-recycling repeated fermentation, minimal medium	83^b	0.87	2.31	[10]
BA305	Sugarcane bagasse (not mentioned)	One stage, anaerobic fed-batch fermentation, complex medium	39.3	0.97	0.33	[58]
DC1515	Corn stalk (enzymatic hydrolysis)	One stage, anaerobic batch fermentation, complex medium	38.6	0.39	0.80	[59]
Anaerobiospirillum succiniciproducens	Oak wood (enzymatic hydrolysis)	One stage, anaerobic batch fermentation, complex medium	24	0.88	0.75	[60]
Mannheimia succiniciproducens	Oak wood (enzymatic hydrolysis)	One stage, anaerobic batch fermentation, complex medium	11.7	0.56	1.17	[61]
Basfia succiniciproducens	Corn stover (dilute acid hydrolysis)	One stage, anaerobic batch fermentation, complex medium	30	0.69	0.43	[62]
Corynebacterium crenatum	Wheat bran (dilute acid hydrolysis)	Two stages, aerobic culture of biomass and anaerobic batch fermentation, Cell-recycling repeated fermentation, minimal medium	43.6	0.59	4.36	[63]
Corynebacterium glutamicum
NC-2	Corn Cob (dilute acid hydrolysis)	Two-stage, aerobic culture of biomass and anaerobic batch fermentation, minimal medium	40.8	0.69	0.85	[18]
CGS5	Corn stalk (enzymatic hydrolysis)	Two-stage, aerobic culture of biomass and anaerobic batch fermentation, minimal medium	98.6	0.87^c	4.29	This study

^aThis yield was defined as the amount of succinic acid produced per substrate consumed
^bThe titer of 83 g L⁻¹ was calculated by summing up the titers of three repetitive fermentations
^cThis yield was calculated based on the consumed glucose, xylose, and citrate

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