Fig. 2

Transcriptional analyses of target genes in mutant strains. qRT-PCR analysis of the relative expression of Trste20 (a), Trsho1 (b), Trsln1 (c) and Trypd1 (d) in the parental and the mutant strains were performed under non-induced (1% glycerol) and induced (1% Avicel) conditions with and without copper. Error bars are SD from three biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001