Table 2 K. pneumoniae strains and plasmids used in this study
Strain or plasmid | Description | Source |
|---|---|---|
Strains | ||
KMK-01 | KCTC 2242 ΔwabG | [12] |
KMK-02 | KCTC 2242 ΔwabG ΔldhA | [12] |
KMK-05 | KCTC 2242 ΔwabG ΔldhA ΔpflB | [12] |
KMK-12 | KCTC 2242 ΔwabG ΔldhA ΔpflB ΔbudA | This study |
KMK-21 | KCTC 2242 ΔwabG ΔldhA ΔpflB ΔbudA ΔdhaD | This study |
KMK-22 | KCTC 2242 ΔwabG ΔldhA ΔpflB ΔbudA ΔglpK | This study |
KMK-23 | KCTC 2242 ΔwabG ΔldhA ΔpflB ΔbudA ΔdhaD ΔglpK | This study |
KMK-23M | KMK-23 edited 5′-UTR of mtlA for reduced expression level | This study |
KMK-46 | KMK-23M ΔdhaKLM | This study |
KMY | KMK-46 harboring pZS21dhaL | This study |
Plasmids | ||
pRedET | λ phage red γ,β,α-producing vector, pBAD_promoter; ori101 Tetr | Gene bridges |
707-FLP | Flp recombinase-producing vector; Psc101 ori cI1578 Tetr | Gene bridges |
pKD4 | FRT-flanked resistance cassette-involved vector; oriRγ Kmr | [16] |
pZA31::MCS | Expression vector, CmR, p15A ori | Expressys |
pZS21::MCS | Expression vector, KmR, pSC101 ori | Expressys |
pZA-Cas9 | pZA31MCS, but PLtetO-1::cas9-PsacB::sacB | [23] |
pZS-CRISPR | pZS21MCS, but PLtetO-1::TracerRNA-PsacB::sacB | [23] |
pZS-CRISPR mtlA | pZS21MCS, but PLtetO-1::TracerRNA-5′UTR(mtlA)-PsacB::sacB | This study |
pZS21dhaL | pZS21::MCS harboring dhaL from wild-type K. pneumoniae | This study |
pZA31budABC | pZA31::MCS harboring budABC from wild-type K. pneumoniae | This study |