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Table 2 K. pneumoniae strains and plasmids used in this study

From: High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae

Strain or plasmid Description Source
Strains
 KMK-01 KCTC 2242 ΔwabG [12]
 KMK-02 KCTC 2242 ΔwabG ΔldhA [12]
 KMK-05 KCTC 2242 ΔwabG ΔldhA ΔpflB [12]
 KMK-12 KCTC 2242 ΔwabG ΔldhA ΔpflB ΔbudA This study
 KMK-21 KCTC 2242 ΔwabG ΔldhA ΔpflB ΔbudA ΔdhaD This study
 KMK-22 KCTC 2242 ΔwabG ΔldhA ΔpflB ΔbudA ΔglpK This study
 KMK-23 KCTC 2242 ΔwabG ΔldhA ΔpflB ΔbudA ΔdhaD ΔglpK This study
 KMK-23M KMK-23 edited 5′-UTR of mtlA for reduced expression level This study
 KMK-46 KMK-23M ΔdhaKLM This study
 KMY KMK-46 harboring pZS21dhaL This study
Plasmids
 pRedET λ phage red γ,β,α-producing vector, pBAD_promoter; ori101 Tetr Gene bridges
 707-FLP Flp recombinase-producing vector; Psc101 ori cI1578 Tetr Gene bridges
 pKD4 FRT-flanked resistance cassette-involved vector; oriRγ Kmr [16]
 pZA31::MCS Expression vector, CmR, p15A ori Expressys
 pZS21::MCS Expression vector, KmR, pSC101 ori Expressys
 pZA-Cas9 pZA31MCS, but PLtetO-1::cas9-PsacB::sacB [23]
 pZS-CRISPR pZS21MCS, but PLtetO-1::TracerRNA-PsacB::sacB [23]
 pZS-CRISPR mtlA pZS21MCS, but PLtetO-1::TracerRNA-5′UTR(mtlA)-PsacB::sacB This study
 pZS21dhaL pZS21::MCS harboring dhaL from wild-type K. pneumoniae This study
 pZA31budABC pZA31::MCS harboring budABC from wild-type K. pneumoniae This study
  1. ApR ampicillin, TetR tetracycline, CmR chloramphenicol, KmR kanamycin resistance