Skip to main content

Table 3 OD600, glycerol consumption and metabolite production yields in K. pneumoniae wild type and engineered strains after 24 h of flask culturea

From: High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae

ldhA

 

Δ

Δ

Δ

pflB

  

Δ

Δ

budA

   

Δ

Strain name

KMK-01

KMK-02

KMK-05

KMK-12

OD600

9.66

12.18

6.47

3.65

Consumed glycerol (g L−1)

48.31

54.45

52.55

16.13

Acetate production (g L−1)

1.42

1.51

0.00

1.34

Succinate production (g L−1)

0.87

0.84

0.82

0.36

Lactate production (g L−1)

2.89

0.00

0.59

0.00

2,3-BDO production (g L−1)

6.00

6.65

11.57

0.60

1,3-PDO production (g L−1)

17.94

20.14

20.11

6.30

1,3-PDO productivity (g L−1 h−1)

0.75

0.84

0.84

0.26

1,3-PDO yield (mol mol−1)b

0.45

0.45

0.46

0.47

  1. a Experiments were repeated three times independently
  2. b Yield was calculated as produced metabolite (mol)/consumed glycerol (mol)