Skip to main content

Table 3 OD600, glycerol consumption and metabolite production yields in K. pneumoniae wild type and engineered strains after 24 h of flask culturea

From: High-yield production of 1,3-propanediol from glycerol by metabolically engineered Klebsiella pneumoniae

ldhA   Δ Δ Δ
pflB    Δ Δ
budA     Δ
Strain name KMK-01 KMK-02 KMK-05 KMK-12
OD600 9.66 12.18 6.47 3.65
Consumed glycerol (g L−1) 48.31 54.45 52.55 16.13
Acetate production (g L−1) 1.42 1.51 0.00 1.34
Succinate production (g L−1) 0.87 0.84 0.82 0.36
Lactate production (g L−1) 2.89 0.00 0.59 0.00
2,3-BDO production (g L−1) 6.00 6.65 11.57 0.60
1,3-PDO production (g L−1) 17.94 20.14 20.11 6.30
1,3-PDO productivity (g L−1 h−1) 0.75 0.84 0.84 0.26
1,3-PDO yield (mol mol−1)b 0.45 0.45 0.46 0.47
  1. a Experiments were repeated three times independently
  2. b Yield was calculated as produced metabolite (mol)/consumed glycerol (mol)