Table 2 Final distribution of Cel7A activity after hydrolysis of nRPS and alkaline washing using different cellulase mixtures
Initial activity (IU/mL) | Celluclast | Accellerase | Cellic | |||
|---|---|---|---|---|---|---|
7.837 ± 0.341 | 18.107 ± 0.102 | 15.003 ± 0.411 | ||||
Activity level (IU/mL) | Fraction (%)b | Activity level (IU/mL) | Fraction (%)b | Activity level (IU/mL) | Fraction (%)b | |
Activity after hydrolysis | ||||||
Liquid | 4.566 ± 0.508 | 61.3 | 12.646 ± 0.361 | 62.9 | 6.031 ± 0.100 | 40.1 |
Solid | 2.077 ± 0.121 | 38.7 | 5.391 ± 0.056 | 37.1 | 6.519 ± 0.596 | 59.9 |
Alkaline washing | ||||||
Liquid | 1.381 ± 0.077 | 60.2 | 4.281 ± 0.038 | 52.5 | 4.194 ± 0.081 | 41.2 |
Solid | 0.651 ± 0.071 | 39.8 | 2.791 ± 0.020 | 47.5 | 4.331 ± 0.125 | 58.8 |
Overall recovery (%)a | 87.9 | 80.8 | 60.2 | |||