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Table 2 Final distribution of Cel7A activity after hydrolysis of nRPS and alkaline washing using different cellulase mixtures

From: Determinants on an efficient cellulase recycling process for the production of bioethanol from recycled paper sludge under high solid loadings

Initial activity (IU/mL) Celluclast Accellerase Cellic
7.837 ± 0.341 18.107 ± 0.102 15.003 ± 0.411
Activity level (IU/mL) Fraction (%)b Activity level (IU/mL) Fraction (%)b Activity level (IU/mL) Fraction (%)b
Activity after hydrolysis
 Liquid 4.566 ± 0.508 61.3 12.646 ± 0.361 62.9 6.031 ± 0.100 40.1
 Solid 2.077 ± 0.121 38.7 5.391 ± 0.056 37.1 6.519 ± 0.596 59.9
Alkaline washing
 Liquid 1.381 ± 0.077 60.2 4.281 ± 0.038 52.5 4.194 ± 0.081 41.2
 Solid 0.651 ± 0.071 39.8 2.791 ± 0.020 47.5 4.331 ± 0.125 58.8
Overall recovery (%)a 87.9 80.8 60.2
  1. Hydrolysis was conducted for 96 h with 18% solids and 20 FPU/gcellulose at 40 °C
  2. aRefers to the sum of the free enzymes on the liquid phase after hydrolysis and alkaline elution
  3. bRefers to the fraction of the total number of IUs found on each fraction