Strains and plasmids | Relevant properties | Source or reference |
---|---|---|
Strains | ||
E. coli TOP10 | F-mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 | Invitrogen |
W023 | W ΔldhA ΔpflB ΔadhE ΔlpdA::K.p. lpdE354 K Δmdh ΔarcA gltAR164L | |
Lactobacillus citreum | Source for ldhD | KCTC3721 |
Lactococcus lactis subsp. lactis | Source for aldB | KCTC3899 |
Saccharomyces cerevisiae S288c | Source for bdh1 | ATCC 204508 |
Plasmids | ||
pUCM | Cloning vector modified from pUC19; constitutive lac promoter, ApR | [23] |
pUCM-gfpm | Constitutive expressed gfpm gene with lac promoter | This study |
pUCN | Cloning/expression vector having inducible wild-type nar promoter, AmpR | [13] |
pUCN-gfpm | Inducible expressed gfpm gene with nar promoter | This study |
pQE-gfpm | Inducible expressed gfpm gene with T5 promoter | [22] |
pUCNr | Cloning/expression vector having rop gene and wild-type nar promoter, AmpR | This study |
pUCNrS | Cloning/expression vector having rop gene and strong nar promoter (S3-2-64), AmpR | This study |
pUCNrI | Cloning/expression vector having rop gene and intermediate nar promoter (W2U-30), AmpR | This study |
pUCNrW | Cloning/expression vector having rop gene and weak nar promoter (W2L-29), AmpR | This study |
NrSL | Inducible expressed ldhD gene from L. citreum on pUCNrS | This study |
NrIL | Inducible expressed ldhD gene from L. citreum on pUCNrI | This study |
NrWL | Inducible expressed ldhD gene from L. citreum on pUCNrW | This study |
NrSi | Inducible expressed ilvBN gene from E. coli on pUCNrS | This study |
NrIi | Inducible expressed ilvBN gene from E. coli on pUCNrI | This study |
NrWi | Inducible expressed ilvBN gene from E. coli on pUCNrW | This study |
NrSa | Inducible expressed aldB gene from L. lactis with strong nar promoter | This study |
NrIa | Inducible expressed aldB gene from L. lactis with intermediate nar promoter | This study |
NrWa | Inducible expressed aldB gene from L. lactis with weak nar promoter | This study |
NrSb | Inducible expressed bdh1 gene from S. cerevisiae with strong nar promoter | This study |
NrIb | Inducible expressed bdh1 gene from S. cerevisiae with intermediate nar promoter | This study |
NrWb | Inducible expressed bdh1 gene from S. cerevisiae with weak nar promoter | This study |
pSTVM2 | Cloning/expression vector removing lac promoter, CmR | [13] |
pSTVM2- SNPL-gfpm | gfpm expression vector with synthetic nar promoter library | This study |
pSTVM-gfpm | Constitutive expressed gfpm gene with constitutive lac promoter on pSTVM2 | This study |
Ni | Inducible expressed ilvBN gene with wild-type nar promoter on pSTVM2 | [13] |
Si | Inducible expressed ilvBN gene with strong nar promoter on pSTVM2 | This study |
Ii | Inducible expressed ilvBN gene with intermediate nar promoter on pSTVM2 | This study |
Wi | Inducible expressed ilvBN gene with weak nar promoter on pSTVM2 | This study |
NiNa | Individually inducible expressed ilvBN and aldB genes with wild-type nar promoter on pSTVM2 | [13] |
SiSa | Individually inducible expressed ilvBN and aldB genes with strong nar promoter on pSTVM2 | This study |
IiIa | Individually inducible expressed ilvBN and aldB genes with intermediate nar promoter on pSTVM2 | This study |
SiWa | Individually inducible expressed ilvBN with strong promoter and aldB with weak nar promoter on pSTVM2 | This study |
NNN | Inducible expressed ilvBN, aldB, and bdh1 genes under each nar promoter on pSTVM2 | [13] |
SWS | Individually inducible expressed ilvBN with strong promoter, aldB with weak promoter, and bdh1 with strong promoter | This study |