Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli

Strains and plasmids

Relevant properties

Source or reference

Strains

 E. coli TOP10

F-mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1

Invitrogen

 W023

W ΔldhA ΔpflB ΔadhE ΔlpdA::K.p. lpdE354 K Δmdh ΔarcA gltAR164L

[13, 21]

 Lactobacillus citreum

Source for ldhD

KCTC3721

 Lactococcus lactis subsp. lactis

Source for aldB

KCTC3899

 Saccharomyces cerevisiae S288c

Source for bdh1

ATCC 204508

Plasmids

 pUCM

Cloning vector modified from pUC19; constitutive lac promoter, ApR

[23]

 pUCM-gfpm

Constitutive expressed gfpm gene with lac promoter

This study

 pUCN

Cloning/expression vector having inducible wild-type nar promoter, AmpR

[13]

 pUCN-gfpm

Inducible expressed gfpm gene with nar promoter

This study

 pQE-gfpm

Inducible expressed gfpm gene with T5 promoter

[22]

 pUCNr

Cloning/expression vector having rop gene and wild-type nar promoter, AmpR

This study

 pUCNrS

Cloning/expression vector having rop gene and strong nar promoter (S3-2-64), AmpR

This study

 pUCNrI

Cloning/expression vector having rop gene and intermediate nar promoter (W2U-30), AmpR

This study

 pUCNrW

Cloning/expression vector having rop gene and weak nar promoter (W2L-29), AmpR

This study

 NrSL

Inducible expressed ldhD gene from L. citreum on pUCNrS

This study

 NrIL

Inducible expressed ldhD gene from L. citreum on pUCNrI

This study

 NrWL

Inducible expressed ldhD gene from L. citreum on pUCNrW

This study

 NrSi

Inducible expressed ilvBN gene from E. coli on pUCNrS

This study

 NrIi

Inducible expressed ilvBN gene from E. coli on pUCNrI

This study

 NrWi

Inducible expressed ilvBN gene from E. coli on pUCNrW

This study

 NrSa

Inducible expressed aldB gene from L. lactis with strong nar promoter

This study

 NrIa

Inducible expressed aldB gene from L. lactis with intermediate nar promoter

This study

 NrWa

Inducible expressed aldB gene from L. lactis with weak nar promoter

This study

 NrSb

Inducible expressed bdh1 gene from S. cerevisiae with strong nar promoter

This study

 NrIb

Inducible expressed bdh1 gene from S. cerevisiae with intermediate nar promoter

This study

 NrWb

Inducible expressed bdh1 gene from S. cerevisiae with weak nar promoter

This study

 pSTVM2

Cloning/expression vector removing lac promoter, CmR

[13]

 pSTVM2- SNPL-gfpm

gfpm expression vector with synthetic nar promoter library

This study

 pSTVM-gfpm

Constitutive expressed gfpm gene with constitutive lac promoter on pSTVM2

This study

 Ni

Inducible expressed ilvBN gene with wild-type nar promoter on pSTVM2

[13]

 Si

Inducible expressed ilvBN gene with strong nar promoter on pSTVM2

This study

 Ii

Inducible expressed ilvBN gene with intermediate nar promoter on pSTVM2

This study

 Wi

Inducible expressed ilvBN gene with weak nar promoter on pSTVM2

This study

 NiNa

Individually inducible expressed ilvBN and aldB genes with wild-type nar promoter on pSTVM2

[13]

 SiSa

Individually inducible expressed ilvBN and aldB genes with strong nar promoter on pSTVM2

This study

 IiIa

Individually inducible expressed ilvBN and aldB genes with intermediate nar promoter on pSTVM2

This study

 SiWa

Individually inducible expressed ilvBN with strong promoter and aldB with weak nar promoter on pSTVM2

This study

 NNN

Inducible expressed ilvBN, aldB, and bdh1 genes under each nar promoter on pSTVM2

[13]

 SWS

Individually inducible expressed ilvBN with strong promoter, aldB with weak promoter, and bdh1 with strong promoter

This study