Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli

Strains and plasmids Relevant properties Source or reference
Strains
 E. coli TOP10 F-mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 Invitrogen
 W023 W ΔldhA ΔpflB ΔadhE ΔlpdA::K.p. lpdE354 K Δmdh ΔarcA gltAR164L [13, 21]
 Lactobacillus citreum Source for ldhD KCTC3721
 Lactococcus lactis subsp. lactis Source for aldB KCTC3899
 Saccharomyces cerevisiae S288c Source for bdh1 ATCC 204508
Plasmids
 pUCM Cloning vector modified from pUC19; constitutive lac promoter, ApR [23]
 pUCM-gfpm Constitutive expressed gfpm gene with lac promoter This study
 pUCN Cloning/expression vector having inducible wild-type nar promoter, AmpR [13]
 pUCN-gfpm Inducible expressed gfpm gene with nar promoter This study
 pQE-gfpm Inducible expressed gfpm gene with T5 promoter [22]
 pUCNr Cloning/expression vector having rop gene and wild-type nar promoter, AmpR This study
 pUCNrS Cloning/expression vector having rop gene and strong nar promoter (S3-2-64), AmpR This study
 pUCNrI Cloning/expression vector having rop gene and intermediate nar promoter (W2U-30), AmpR This study
 pUCNrW Cloning/expression vector having rop gene and weak nar promoter (W2L-29), AmpR This study
 NrSL Inducible expressed ldhD gene from L. citreum on pUCNrS This study
 NrIL Inducible expressed ldhD gene from L. citreum on pUCNrI This study
 NrWL Inducible expressed ldhD gene from L. citreum on pUCNrW This study
 NrSi Inducible expressed ilvBN gene from E. coli on pUCNrS This study
 NrIi Inducible expressed ilvBN gene from E. coli on pUCNrI This study
 NrWi Inducible expressed ilvBN gene from E. coli on pUCNrW This study
 NrSa Inducible expressed aldB gene from L. lactis with strong nar promoter This study
 NrIa Inducible expressed aldB gene from L. lactis with intermediate nar promoter This study
 NrWa Inducible expressed aldB gene from L. lactis with weak nar promoter This study
 NrSb Inducible expressed bdh1 gene from S. cerevisiae with strong nar promoter This study
 NrIb Inducible expressed bdh1 gene from S. cerevisiae with intermediate nar promoter This study
 NrWb Inducible expressed bdh1 gene from S. cerevisiae with weak nar promoter This study
 pSTVM2 Cloning/expression vector removing lac promoter, CmR [13]
 pSTVM2- SNPL-gfpm gfpm expression vector with synthetic nar promoter library This study
 pSTVM-gfpm Constitutive expressed gfpm gene with constitutive lac promoter on pSTVM2 This study
 Ni Inducible expressed ilvBN gene with wild-type nar promoter on pSTVM2 [13]
 Si Inducible expressed ilvBN gene with strong nar promoter on pSTVM2 This study
 Ii Inducible expressed ilvBN gene with intermediate nar promoter on pSTVM2 This study
 Wi Inducible expressed ilvBN gene with weak nar promoter on pSTVM2 This study
 NiNa Individually inducible expressed ilvBN and aldB genes with wild-type nar promoter on pSTVM2 [13]
 SiSa Individually inducible expressed ilvBN and aldB genes with strong nar promoter on pSTVM2 This study
 IiIa Individually inducible expressed ilvBN and aldB genes with intermediate nar promoter on pSTVM2 This study
 SiWa Individually inducible expressed ilvBN with strong promoter and aldB with weak nar promoter on pSTVM2 This study
 NNN Inducible expressed ilvBN, aldB, and bdh1 genes under each nar promoter on pSTVM2 [13]
 SWS Individually inducible expressed ilvBN with strong promoter, aldB with weak promoter, and bdh1 with strong promoter This study