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Table 2 Primers used in this study

From: Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli

Primer name

Sequence (5′–3′)

For cloning

 XmaI-SNPL-gfpm-F

TCCCCCCGGGCTCTTGATCGTTATCAAATCCCANNNNNNNNNNNNNNNGTATAATGCCCTTAAAAGGAGGATTACAAAATGAGTAAAGGAGAAGAACT

 SphI-gfpm-R

ACATGCATGCTTAGTGGTGGTGGTGGTGGTGTTTGTAGAGCTCATCGATGC

 XmaI-gfpm-F

TCCCCCCGGGAGGAGGATTACAAAATGAGTAAAGGAGAAGAACTTTT

 NotI-gfpm-R

TAAGAATGCGGCCGCTTAGTGGTGGTGGTGGTGGTGTTTGTAGAGCTCATCGATGC

 ldhD-citreum-F-XbaI

CTAGTCTAGAAGGAGGATTACAAAATGAAGATTTTTGCTTATGGT

 ldhD-citreum-R-NotI

TTCCCTTGCGGCCGCTTAATACTTTACAGCAATACTT

 ilvB-EC-F-XbaI

CTAGTCTAGAAGGAGGATTACAAAATGGCAAGTTCGGGCA

 ilvN-EC-R-NotI

TTCCCTTGCGGCCGCTTACTGAAAAAACACCGCGAT

 aldB-LL-F-XbaI

CTAGTCTAGAAGGAGGATTACAAAATGACAGAAATCACACAACTT

 aldB-LL-R-NotI

TTCCCTTGCGGCCGCTCATTCAGCTACATCGATATC

 bdh1-SC-F-XmaI

TCCCCCCGGGAGGAGGATTACAAAATGAGAGCTTTGGCATATTTC

 bdh1-SC-R-NotI

TTCCCTTGCGGCCGCTTACTTCATTTCACCGTGATT

 SphI-pUC-F

ACATGCATGCCCGACTGGAAAGCG

 SphI-pUC-R

ACATGCATGCCGGTGTGAAATACCG

 PstI-pUC-F

AAAACTGCAGCCGACTGGAAAGCG

 BamHI-pUC-R

CGGGATCCCGGTGTGAAATACCG

 PnarS-R

TCTCTAACACAGTATTGGGATTTGATAACGATCAAG

 PnarI-R

AATCTAGTAACCCGATGGGATTTGATAACGATCAAG

 PnarW-R

GGAGATGTTACAATATGGGATTTGATAACGATCAAG

 -10Pnar-F

GTATAATGCCCTTAAATCTAGA

 pUCN-ori-fr

AGGAAGCGGAAGAGCG

 pUCN-ori-r

GAAGATCCTTTGATCTTTTCTA

 pET-ori-R

TTGAGATCCTTTTTTTCTGC

 pET-rop-F

GGTGCGCATGATCGTG

 pSTVM2-pUC-sub-USER-3-F

AGACAGUCATAAGTGCGG

 pSTVM2-pUC-sub-USER-1-R

ATGCAACUCGTAGGACAG

 pUC-sub-USER-1-F

AGTTGCAUCCCGACTGGAAAGCG

 pUC-sub-USER-2-F

ATCCATGUCCCGACTGGAAAGCG

 pUC-sub-USER-5-F

ATATGCGAUCCCGACTGGAAAGCG

 pUC-sub-USER-2-R

ACATGGAUATGCGGTGTGAAATACC

 pUC-sub-USER-5-R

ATCGCATAUATGCGGTGTGAAATACCG

 pUC-sub-USER-3-R

ACTGTCUATGCGGTGTGAAATACCG

For qRT-PCR

 q-cysG-F

TTGTCGGCGGTGGTGATGTC

 q-cysG-R

ATGCGGTGAACTGTGGAATAAACG

 q-gfpm-F

AGAGGGTGAAGGTGATGCCA

 q-gfpm-R

AGATGATCCGGATAACGCGC

  1. Italic and underline letters represent a restriction enzyme site