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Table 1 Selective removal of xylan by dilute sulfuric acid pretreatment of wheat straw under mild condition and the CAC determined by His6–CBM–GFP2 probe protein based on Langmuir adsorption

From: Visualizing cellulase adsorption and quantitatively determining cellulose accessibility with an updated fungal cellulose-binding module-based fluorescent probe protein

H2SO4 con. (wt%) Contents of polymeric components Fitted Langmuir equation parameters and CAC
Gln (%) Xln (%) Lig (%) Amax (μg/mg) Kp R 2 CAC (m2/g)
MCC 47.1 ± 19.2 0.00652 ± 0.00417 0.8721 9.3 ± 3.8
Raw wheat straw 35.1 ± 1.4 25.0 ± 0.8 27.9 ± 1.5 3.1 ± 0.6 0.00332 ± 0.00114 0.9547 0.6 ± 0.1
0.4 49.9 ± 0.2 17.0 ± 0.6 30.4 ± 0.4 12.2 ± 1.12 0.00157 ± 0.00032 0.9904 2.4 ± 0.2
1 55.2 ± 0.9 10.5 ± 0.2 31.3 ± 0.3 21.5 ± 2.9 0.00119 ± 0.00042 0.9806 4.3 ± 0.6
3 56.5 ± 1.1 5.01 ± 0.3 33.4 ± 0.3 33.8 ± 4.0 0.00071 ± 0.00031 0.9865 6.7 ± 0.8
5 62.9 ± 0.7 3.81 ± 0.2 32.5 ± 1.6 27.5 ± 3.5 0.00085 ± 0.00032 0.9851 5.5 ± 0.7
  1. Samples were first treated by 5 g/L BSA to block the nonspecific adsorption of the probe protein on lignin
  2. Gln glucan (cellulose) content, Xyl xylan content, Lig lignin content