Skip to main content

Table 1 Selective removal of xylan by dilute sulfuric acid pretreatment of wheat straw under mild condition and the CAC determined by His6–CBM–GFP2 probe protein based on Langmuir adsorption

From: Visualizing cellulase adsorption and quantitatively determining cellulose accessibility with an updated fungal cellulose-binding module-based fluorescent probe protein

H2SO4 con. (wt%)

Contents of polymeric components

Fitted Langmuir equation parameters and CAC

Gln (%)

Xln (%)

Lig (%)

Amax (μg/mg)

Kp

R 2

CAC (m2/g)

MCC

47.1 ± 19.2

0.00652 ± 0.00417

0.8721

9.3 ± 3.8

Raw wheat straw

35.1 ± 1.4

25.0 ± 0.8

27.9 ± 1.5

3.1 ± 0.6

0.00332 ± 0.00114

0.9547

0.6 ± 0.1

0.4

49.9 ± 0.2

17.0 ± 0.6

30.4 ± 0.4

12.2 ± 1.12

0.00157 ± 0.00032

0.9904

2.4 ± 0.2

1

55.2 ± 0.9

10.5 ± 0.2

31.3 ± 0.3

21.5 ± 2.9

0.00119 ± 0.00042

0.9806

4.3 ± 0.6

3

56.5 ± 1.1

5.01 ± 0.3

33.4 ± 0.3

33.8 ± 4.0

0.00071 ± 0.00031

0.9865

6.7 ± 0.8

5

62.9 ± 0.7

3.81 ± 0.2

32.5 ± 1.6

27.5 ± 3.5

0.00085 ± 0.00032

0.9851

5.5 ± 0.7

  1. Samples were first treated by 5 g/L BSA to block the nonspecific adsorption of the probe protein on lignin
  2. Gln glucan (cellulose) content, Xyl xylan content, Lig lignin content