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Table 1 Summary of the experimental set-up used for the processing of a lactate-rich medium to methane

From: Methane-yielding microbial communities processing lactate-rich substrates: a piece of the anaerobic digestion puzzle

Methane-yielding microbial communities processing lactate-rich medium to methane

 

M1A

M1B

The seed methanogenic inoculum

Activated sludge from a municipal waste treatment plant “Warszawa Południe” in Warsaw, Poland, sampled in the wintera

Methane-yielding sludge from the 50-L-UASB bioreactor processing acidic effluent from molasses fermentation [19] inoculated with activated sludge from a municipal waste treatment plant “Warszawa Południe” in Warsaw, Poland, sampled in the autumna

Lactate-rich medium—modified M9 (containing MgCl2 instead of MgSO4, without glucose)

Sodium lactate 8.26 g/L, butyric acid 1.06 g/L, propionic acid 0.97 g/L, acetic acid 1.54 g/L

Sodium lactate 7 g/L, sodium butyrate 1.3 g/L, propionic acid 0.99 g/L, acetic acid 1.05 g/L, yeast extract 0.5 g/L

Hydraulic retention time (HRT), days

7

7

Culture history

- Incubation at room temperature (20–25 °C) for 26 days after inoculation

- 27th–57th day of cultivation—neutralized medium continuously supplied to the bioreactor

- Since 58th day of cultivation—non-neutralized medium supplied to the bioreactor

Incubation at room temperature (20–25 °C) for 10 days after inoculation.

Since 11th day of cultivation—non-neutralized medium supplied to the bioreactor

Sample collection for 16S rRNA profiling

37th week of cultivation

40th week of cultivation

Analyses performed on the samples collected from the UASB reactors shown in Table 2

33rd—40th week of cultivation

35th—40th week of cultivation

Sample collection for isotope analyses of fermentation gas

44th—50th week of cultivation

  1. aThe director of the Municipal Water and Sewage Enterprise in the capital city of Warsaw in Poland issued the permission to sample activated sludge and use it for scientific research