Fig. 3

Distribution of selected components in HBY31 cells obtained by confocal imaging via Raman spectroscopy. The cell area was visualized through the integration of: a all bands in the fingerprint region (‘Organic matter’, 1800–400 cm⁻¹) and b integration of the band corresponding to ν_s(CH₂) of proteins (‘Proteins’, 2925 cm⁻¹). LBs can be visualised by integration of lipid-related bands corresponding to: c δ(CH₂) in saturated fatty acids (‘SFA’, 1444 cm⁻¹), d ν(C=C) in unsaturated fatty acids (‘UFA’, 1656 cm⁻¹) and e ν(C=O) in triglycerides (‘TAG’, 1740 cm⁻¹). The obtained results enable not only to visualise LBs, but also to demonstrate the presence of SFA, UFA and TAG specifically within those structures, as well as show homogenous distribution of proteins in the cytoplasm. Size of the imaged area: 12.86 × 12.36 µm