Fig. 1
From: Engineering global transcription to tune lipophilic properties in Yarrowia lipolytica
Concept of global transcription engineering in Y. lipolytica. a Spt15 is a key transcription factor (TF) in TFIID of S. cerevisiae. By BLAST analysis, we got a Spt15-like protein named as Yl-Spt15 coded by the gene YALIOB23056g. This Yl-Spt15 was probable to take the similar role as Spt15. b The manipulated Yl-SPT15 mutant libraries were inserted in five expression cassettes and assembled meanwhile integrated in yeast chromosomal GUT2 site by in vivo homologous recombination. The cells obtaining different mutant combinations were screened and selected