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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Engineering global transcription to tune lipophilic properties in Yarrowia lipolytica

Fig. 5

Effects of single native gene overexpression. a The genes associated with up-regulated pathway of ketone body metabolism in Yl_5_2. The numbers under genes are log2(fold change of transcriptional read count). b The genes associated with up-regulated alpha-linolenic acid metabolism in Yl_5_2. However, it did not exist in Y. lipolytica. The actual functions of the coupling of ACX and POT1 shall be to produce final acetyl-CoA. Another gene TGL4 charged degradation of multiple compounds to recycle free fatty acids. The numbers under genes are log2(fold change of transcriptional read count). c Seven typical genes were chosen from the up-regulated pathways in Yl_5_2 (relative to Yl_5_1) and transformed as one more cassette into the same strain. The results showed that all the seven transformed strains with recombinant plasmids gained higher beta-carotene production than the initial strain Yl_5_2 with blank plasmid (Yl_5_2_0). All error bars indicate ± standard deviation, n = 3

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