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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Mannanase hydrolysis of spruce galactoglucomannan focusing on the influence of acetylation on enzymatic mannan degradation

Fig. 2

Temporal pattern showing the increase in reducing sugar equivalents over 24 h when applying CjMan5A or CjMan26A on KGMN (a) and LBGN (d). The amounts of mannooligosaccharides produced (M1–M4) at three times during hydrolysis with either CjMan5A (b and e) or CjMan26A (c and f) on KGMN (upper panel) and LBGN (lower panel) are also shown. The hydrolysis reactions analyzed in terms of reducing sugars contained 10 nM enzyme and 0.1% (w/v) mannan substrate. The reaction times and enzyme concentrations used for the quantification of mannooligosaccharides using HPAEC-PAD were for CjMan5A reactions: 10 min, 25 nM, 30 min, 25 nM, and 24 h, 100 nM; and for CjMan26A reactions: 5 min, 10 nM, 30 min, 25 nM, and 24 h, 100 nM. The high enzyme concentration of 100 nM was used for 24-h reactions to ensure complete hydrolysis. Error bars signify standard errors of the mean of duplicate HPAEC-PAD measurements or triplicate reducing sugar measurements

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